一株浒苔拉乌尔菌HT15-8的菌种鉴定及其抗肿瘤活性初步研究

Identification of a Strain of Lactobacillus enteritidis HT15-8 and Preliminary Study on Its Antitumor Activity

目的:鉴定浒苔共生微生物HT15-8的菌种,同时对其代谢产物进行初步的分离纯化和抗肿瘤活性研究。方法:发酵培养10株浒苔共生微生物,用细胞毒活性检测法分别对其代谢产物进行抗肿瘤活性的筛选。选取活性最好的菌株HT15-8进行形态观察、生理生化实验鉴定、16S rRNA基因序列同源性分析。利用倒置显微镜观察HT15-8代谢产物的活性组分对BEL-7402、A549细胞形态的影响。结果:菌株HT15-8属于拉乌尔菌属,倒置显微镜下观察发现,菌株HT15-8代谢产物的活性组分能够明显改变肿瘤细胞形态并最终死亡。结论:浒苔共生微生物HT15-8为拉乌尔菌属,其产生的代谢产物对BEL-7402和A549两种肿瘤细胞具有初步的抗肿瘤活性。

Objective： To identify the species of the symbiotic micro-organism HT15-8 of Brassica oleracea, and to isolate and purify its metabolites. Methods： Ten symbiotic microorganisms of Brassica oleracea were fermented and cultured, and their metabolites were screened for antitumor activity by cytotoxicity assay. The best active strain HT15-8 was selected for morphological observation, physiological and biochemical experimental identification, and 16 S r RNA gene sequence homology analysis. The effect of the active components of HT15-8 metabolites on the morphology of BEL-7402 and A549 cells was observed using an inverted microscope. Results： The strain HT15-8 belonged to the genus Raulia. It was observed under an inverted microscope that the active components of the metabolite HT15-8 significantly changed the morphology of the tumor cells and eventually died. Conclusion： The symbiotic microorganism HT15-8 of Brassica oleracea is a genus Raulia, and its metabolites have a preliminary anti-tumor activity on BEL-7402 and A549 tumor cells.