唾液miRNA-205检测在喉鳞状细胞癌中的诊断价值

Diagnostic value of salivary microRNA-205 in laryngeal squamous cell carcinoma

目的初步探讨唾液miRNA-205检测在喉鳞状细胞癌中的诊断价值。方法收集了于该院就诊的15例喉鳞状细胞癌患者、10例喉息肉患者、10例健康者的唾液标本,使用反转录聚合酶链反应(RT-PCR)定量检测并比较唾液miRNA-205的相对表达情况。结果喉鳞状细胞癌患者的唾液中miRNA-205的表达水平较健康对照者显著上调(P=0.036)。受试者工作特征(ROC)曲线分析miRNA-205对喉鳞状细胞癌的诊断价值发现,唾液中miRNA-205的ROC曲线下面积为0.753 0(95%CI:0.546 0~0.961 0,P=0.036)。结论喉鳞状细胞癌患者唾液中的miRNA-205存在高表达,有望作为早期诊断喉鳞状细胞癌的指标。检测miRNA-205在唾液中的表达,将来有望开启喉鳞状细胞癌早期诊断的无创时代。

Objective To preliminarily explore the diagnostic value of salivary miRNA-205 in laryngeal squamous cell carcinoma （LSCC）. Methods The saliva samples were collected from 15 cases of LSCC,10 cases of laryngeal polyp and 10 healthy controls.The real-time quantitative reverse transcription PCR（RT-PCR） was employed to quantitatively detect and compare the relative expression levels of miRNA-205. Results The expression level of salivary miRNA-205 in the patients with laryngeal squamous cell carcinoma was significantly up-regulated compared with healthy controls （ P =0.036）.The analysis of miRNA205 for diagnosing LSCC by using the ROC curve found that the area under the ROC curve （AUC） of salivary miRNA-205 was 0.753 0 （95% CI ：0.546-0.961, P =0.036）. Conclusion miRNA-205 is highly expressed in saliva of the patients with laryngeal squamous cell carcinoma,and is expected to serve as indicator for diagnosing LSCC.Detecting saliva miRNA-205 expression is likely to open the non-invasive era of LSCC early diagnosis in the future.