PPARγ与MSX2在甲状腺肿瘤术后患者骨代谢的作用机制研究

Study on role of PPARγand MSX2 in bone metabolism after thyroid tumor operation

目的探讨过氧化物酶增殖物受体γ(PPARγ)与MSX2/Hox8.1蛋白(MSX2)在甲状腺肿瘤术后患者骨代谢过程中的作用。方法治疗组29例甲状腺肿瘤术后患者行骨髓穿刺+活检术,对照组为1︰1匹配非甲状腺肿瘤患者。两组进行骨组织HE染色,采用免疫组织化学半定量检测PPARγ与MSX2水平,比较治疗组与对照组之间的差异。结果 HE染色骨组织形态学显示,治疗组脂肪面积明显增多,骨小梁面积及宽度均减少,两组比较差异有统计学意义(t=2.293、2.872,P<0.05);免疫组织化学半定量结果显示,治疗组PPARγ表达量高于对照组,MSX2表达量低于对照组,差异均有统计学意义(P<0.05)。结论 PPARγ与MSX2在甲状腺肿瘤术后骨代谢的过程中起到了重要作用,甲状腺肿瘤术后患者发生骨质疏松症可能与成脂肪作用被促进,成骨作用被抑制相关。

Objective To investigate the role of PPARγ and MSX2/Hox 8.1 protein （MSX2） in bone metabolism process after thyroid tumor operation. Methods Twenty-nine postoperative cases of thyroid tumor in the treatment group conducted bone marrow puncture plus biopsy,and the control group included 1∶1 matched cases of non-thyroid tumor.The two groups conducted the bone tissue HE staining.The immunohistochemical method was adopted to semiquantitatively detect the levels of PPARγ and MSX2.The differences between the treatment group and control group were compared. Results The bone histomorphometry by HE staining showed that visceral fat area in the treatment group was significantly increased,the bone trabecular area and width were decreased,the differences between the two groups were statistically significant （ t = 2.293 , 2.872 , P 〈 0.05）.The immunohistochemical semiquantitative result showed that the PPARγ expression level in the treatment group was higher than that in the control group,while the MSX2 expression level was lower than that in the control group,the difference between the two groups was statistically significant （ P 〈 0.05 ）. Conclusion PPARγ and MSX2 play an important role in bone metabolic process after thyroid tumor operation.The osteoporosis occurrence after operation in the patients with thyroid tumor may be associated with the adipogenesis being promoted and osteogenesis being inhibited.