生理状态下人牙周膜成纤维细胞的原子力显微镜下的观察

Observation of the morphology of living human periodontal ligament cells with atomic force microscope

目的:利用原子力显微镜(AFM)观察人牙周膜细胞(h PDLCs)的表面形貌及细胞骨架。方法:用酶消化组织块法获得原代h PDLCs并经免疫组化鉴定,用弹性常数为0. 03 N/m标准原子力微悬臂进行接触模式扫描,采集细胞原子力显微镜的纳米级高分辨细胞形貌图。结果:h PDLCs波形丝蛋白阳性,角蛋白染色阴性。原子力显微镜图像显示,生理状态下h PDLCs细胞膜下有应力纤维结构。结论:h PDLCs细胞膜下具有应力纤维结构。

AIM： To observe the morphology of living human periodontal ligament cells（hPDLCs） with atomic force microscope（AFM）. METHODS： hPDLCs were primary cultured with tissue block enzymolytic method and identified by immunohistochemisty（IHC）. High resolution images of the morphology of hPDLCs were obtained by contact scanning with standard AFM microcantilever elastic constant of 0.03 N/m. RESULTS ： IHC revealed that hPDLCs werepositive for vimentin and negative for keratin. AFM images of hPDLCs showed fiber structure under stress beneath cell membrane. CONCLUSION：hPDLCs have stress fiber structure beneath cell membrane.