硼替佐米对淋巴瘤细胞增殖凋亡和SHP-2基因表达的影响研究

Effects of Bortezomib on Proliferation,Apoptosis and SHP-2 Gene Expression in Lymphoma Cells

目的观察硼替佐米对淋巴瘤细胞株Jurkat细胞、Raji细胞增殖凋亡和SHP-2基因表达的影响。方法利用淋巴瘤细胞株Jurkat细胞和Raji细胞进行传代培养,用1.0、5.0、25.0和100.0 nmol/L不同浓度的硼替佐米进行处理,观察不同时间段细胞增殖抑制率和凋亡率,比较实验组和对照组SHP-2基因表达水平。结果随着硼替佐米浓度增加,相同干预时间下的Jurkat细胞、Raji细胞抑制率增加;随着干预时间延长,相同硼替佐米浓度时,Jurkat细胞、Raji细胞抑制率也增加。随着硼替佐米浓度增加,相同干预时间下的Jurkat细胞、Raji细胞凋亡率增加;随着干预时间延长,干预浓度相同时,Jurkat细胞、Raji细胞凋亡率也增加。对照组的SHP-2表达量小于硼替佐米干预组,且干预时间相同时,随着浓度增加,SHP-2表达量升高;药物浓度相同,随着干预时间延长,SHP-2表达量升高。结论硼替佐米可抑制淋巴瘤细胞株Jurkat细胞、Raji细胞增殖,促进凋亡并提高SHP-2基因表达量,相关作用与浓度有关。

Objective To observe the effects of bortezomib on apoptosis and expression of SHP-2 gene in the proliferation of lymphoma cell line Jurkat cells and Raji cells.Methods Jurkat cells and Raji cells were used for subculture,and the proliferation inhibition rate and apoptosis rate of 1.0 nmol/L,5.0 nmol/L,25.0 nmol/L and 100.0 nmol/L were observed. The expression level of SHP-2 gene in the experimental group and the control group was compared. Results With the increase of bortezomib concentration,the inhibition rate of Jurkat cells and Raji cells increased within the same intervention time. With the increase of intervention time,the inhibition rate of Jurkat cells and Raji cells also increased at the same bortezomizomi concentration. The expression of SHP-2 in the blank control group was lower than that of the bortezomizomi intervention group. Within the same intervention time,the expression of SHP-2 increased not only with theconcentration,but also with time at the same concentration of drugs.Conclusion Bortezomib significantly affects proliferation,apoptosis and SHP-2 gene expression of lymphoma cell line Jurkat cells and Raji cells in a concentration-dependent manner.