卡介苗对阿尔茨海默病模型小鼠行为和炎症因子的影响

Impact of Bacillus Calmette-Guérin on the behavior and inflammatory factors in Alzheimer disease mouse model

目的:研究卡介苗对阿尔茨海默病(AD)模型小鼠行为及炎症因子水平的影响。方法:将32只成年C57BL/6小鼠随机分成四组,分别为对照组、AD组、卡介苗低剂量组(104CFU)和高剂量组(105CFU)。除对照组外,其他三组小鼠均以连续腹腔注射D-半乳糖建立AD模型,其中两组施以不同剂量的卡介苗。各组小鼠通过旷场和水迷宫检测其活动度和学习记忆功能;免疫荧光检测脑内Aβ斑块; ELISA检测血清中炎症因子TNF-α和IFN-γ的含量。结果:与对照组相比,AD小鼠活动度下降,学习记忆能力退化,脑内可检测到Aβ沉积,血清中TNF-α、IFN-γ含量上升,而经过卡介苗处理的AD小鼠,不论剂量高低,其行为学均得到改善(P<0.05),脑内Aβ斑块减少(P<0.05),血清中TNF-α和IFN-γ含量降低(P<0.05)。结论:低剂量和高剂量的卡介苗均能改善AD小鼠的行为,降低小鼠脑内Aβ沉积,控制血清中的炎症因子。

Objective ：To obsetne the impact of Bacillus Calmette-Guerin （BCG） on the behavior and inflaramatory factors of model mice with Alzheimer disease（AD）. Methods：Thirty-two adult C57BL/6 mice were equally divided into control group, AD group, low BCG （104CFL） and high BCG （ 105 CFL ） treatment groups. AD mouse models were induced by continuous intraperitoneal injection of D-galaetose in three groups except the controls, and different dosage of BCG was applied to two groups of model mice. Then mice in each group were subjected to open-field test and Morris water maze test to observe the activities and the learning and memory abilities, Immunofluoreseenee stain was used to detect amyloid-beta（Aβ）-associated cerebral plaques, and ELISA was performed to measure levels of inflammatory factors TNF-α and IFN-γ Results ： Compared with the controls, AD mice had decreased activi- ty and ability of learning and memory,and Aβ deposition could be observed in the brain with increased serum levels of TNF-α and IFN-γ. l lowever, the mice had improved behaviors as well as decreased deposition of Aβ and TNF-α and IFN-γ levels following treatment with BCG either in low or high dosage （all P〈 0.05）. Conclusion：Either low or high dosage of BCG can improve the behaviors in AD mouse, reduce the Aβ-assoeiated plaques and regulate the serum inflammatory factors.