伯氏嗜木螨线粒体基因组全序列测定研究

Determination of the complete mitochondrial genome of Caloglyphus berlesei(Acaridae:Astigmata)

目的:测定并分析伯氏嗜木螨线粒体基因组全序列。方法:采用长片段PCR扩增和引物步移法测序。结果:伯氏嗜木螨线粒体基因组全序列为14 273 bp,为闭合环状分子,包括13个蛋白质编码基因、22个tRNA和2个rRNA。tRNA长度47～63 bp,平均(53.6±3.49) bp,仅trn K能折叠形成三叶草结构,其余均为缺乏T-臂或D-臂的非典型结构;最大的非编码区LNR位于trn F和trn S1之间,长341 bp,有1段类似微卫星的重复序列(AT) n和多个能稳定形成茎环的二级结构。结论:获得了伯氏嗜木螨线粒体基因组全序列。

Objective： To determine the complete mitoehondrial genome and analyze its sequence of Caloglyphus berlesei （C. berlesei）.Methods：Four long fragments were amplified hy long PCR and sequenced hy conserved primer-walking technique. Results ：The complete mitochondrial genome of C. berlesei was a circular molecule of 14 273bp, consisting of 13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes. Most inferred tRNA genes were extremely truncated [ 47-63 bp, with an average of （53.6 ± 3.49） bp] Stem-loops were absent on either the T- or D-arm except for the trnK. The largest non-coding region （341 bp）was present between wnF and trnS1, and contained a mierosatellite-like （AT）n sequence and putative seeondary structure features. Conclusion：Complete C. berlesei mitoehondrial genome was successfully determined and sequenced.