SERPINA 3在舌鳞状细胞癌组织中表达及临床意义

Expression and clinical implications of SERPINA 3 gene in squamous cell carcinoma of tongue

目的:探讨SERPINA 3在舌鳞状细胞癌组织中表达及临床意义。方法:选取2010年3月～2012年4月在我院口腔科择期行手术治疗的63例舌鳞状细胞癌(TSCC)患者的肿瘤组织,同期留取45例因舌外伤或舌部良性病变行手术切除的正常舌黏膜组织作为对照组,分别利用免疫组化法和实时荧光定量PCR技术检测TSCC和对照组组织中SERPINA 3蛋白和基因表达,生存分析采用Kaplan-Meier法,利用Cox比例风险回归模型分析TSCC患者生存的影响因素。结果:TSCC组织中SERPINA 3蛋白阳性表达率高于对照组,差异有统计学意义(χ~2=15.429,P=0.000); TSCC组织中SERPINA 3 mRNA相对表达量高于对照组,差异有统计学意义(t=44.711,P=0.000); TSCC组织中SERPINA 3蛋白和基因表达与分化程度、TNM分期和颈淋巴结转移有关(P<0.05); Kaplan-Meier生存分析结果显示,SERPINA 3蛋白阳性表达组患者5年总体生存率和平均生存时间均高于阴性表达组,Log-Rank检验差异有统计学意义(χ~2=4.830,P=0.028); Cox比例风险回归模型分析结果显示,SERPINA 3蛋白阳性表达、TNM分期Ⅲ～Ⅳ期、发生颈淋巴结转移是TSCC患者预后的独立影响因素。结论:TSCC组织中SERPINA 3蛋白和基因呈高表达,且与分化程度、TNM分期和颈淋巴结转移有关,是影响患者预后的因素之一。

Objective： To investigate the expression and clinical implications of SERPINA 3 gene in squamous cell carcinoma of tongue （TSCC）. Methods：Sixty-three cases of TSCC undergoing elective surgery in our department were included from March 2010 to April 2012, and not＇real tongue muco- sal tissues were obtained in another 45 patients undergone surgery in the corresponding period due to tongue trauma or tongue benign lesions （ control group）, hnmunohistochemistry and real-time fluorescence quantitative PCR were performed to detect the expression of SERPINA 3 protein and gene in the samples of TSCC and the controls. Kaplan-Meier method was used to assess the survival, and Cox proportional hazards regression model was used to analyze the factors affecting the survival of patients with TSCC. Results ：The positive expression rate of SERPINA 3 was higher in TSCC patients than that in the control group（X^2= 15.429,P=0.000） ,and the relative expressiory level of SERPINA 3 mRNA was also higher in TSCC patients than that of the controls （ t=44.711, P=0.000）. Expression of SERPINA 3 protein and gene was related to the degree of differentiation, TNM stage and cervical lymph node metas- tasis in patients of TSCC （P〈0.05）. Kaplan-Meier survival analysis showed that the overall 5-year survival rate and mean survival year were higher in SER- P INA 3 positive expression group were higher than those in the negative expression group, and difference was significant by Log-rank test（ X2=4.830,P=0.028）. Cox proportional hazards regression model analysis showed that SEItPINA 3 protein positive expression,TNM stage in Ⅲ～Ⅳ,cervital lymph node metastasis were independent factor for estimating the prognosis in patients with TSCC. Conclusion：SERPINA 3 protein and gene are highly expressed in patients of TSCC, and associated with the degree of differentiation,TNM stage and cervical lymph node metastasis,which can be important factors affecting the prognosis of such patients.