丹红注射液对脑缺血再灌注大鼠脑水肿的影响

Efficacy of the Danhong injection on edema after cerebral ischemia reperfusion in rats

目的:研究丹红注射液对脑梗死脑缺血再灌注大鼠脑水肿的影响。方法:采用大鼠大脑中动脉闭塞法制作局灶性脑缺血再灌注模型。应用干-湿比重法测定脑含水量,免疫组化SABC法测定水孔蛋白4(AQP4)的表达,化学比色法测定脑组织中的Na-K-ATP酶活性。结果:模型组AQP4蛋白表达升高;予丹红注射液的治疗组AQP4蛋白的表达下降;模型组脑组织匀浆Na-K-ATP酶活性明显低于正常对照组,予丹红注射液的治疗组则明显高于模型组。结论:脑组织缺血区AQP4蛋白表达的上调和Na-K-ATP酶活性降低可能与脑水肿的发生发展有一定的关系,丹红注射液有抑制脑水肿发生的作用。

Objective： To study the efcacy of the Danhong injection （ 丹红注射液 ） on cerebral edema after cerebral ischemia reperfusion in rats. Methods： The model of focal cerebral ischemia reperfusion was made by middle cerebral artery occlusion method. The content of brain was determined by the dry-wet specifc gravity method. The expression of water pore protein 4 （AQP4） was determined by immunohistochemical SABC method. The activity of NA and K-ATP enzyme in brain tissue was determined by chemical colorimetric method. Results： The expression of AQP4 protein in the model group was elevated. The expression of AQP4 protein in the treatment group was decreased. The activity of Na-K-ATP in the model group was signifcantly lower than that in the control group; and that in the treatment group was signifcantly higher than the model group. Conclusion： The increase of expression of AQP4 protein and the decrease of activity of NA-K-ATP enzyme in cerebral ischemic region may be related to the occurrence and development of brain edema. The Danhong injection can inhibit the occurrence of brain edema.