大鼠比目鱼肌梭内肌细胞钙超载模型的建立

The establishment of intrafusal muscle cell calcium overload model of soleus muscle in rats

应用Ca^(2+)导入剂Ionomycin建立大鼠比目鱼肌梭内肌细胞钙超载模型。实验采用激光扫描共聚焦显微镜技术,观察给予不同浓度Ca^(2+)导入剂Ionomycin后,大鼠比目鱼肌梭内肌细胞内静息Ca^(2+)的变化。实验结果显示给予2μmol/L Ionomycin灌流后,梭内肌细胞内Ca^(2+)浓度缓慢升高(P <0. 01),达峰后(2. 67±0. 43) min,MFI最大增值降低50%;给予5μmol/L Ionomycin灌流后,梭内肌细胞内Ca^(2+)浓度迅速升高(P <0. 01),达峰后(6. 26±0. 87)min,MFI最大增值降低50%。提示给予2μmol/L Ionomycin灌流肌梭可以形成短期的钙超载,给予5μmol/L Ionomycin则可以建立稳定的大鼠比目鱼肌梭内肌细胞钙超载模型。为大鼠肌梭生理的研究及钙离子与失重性肌萎缩的实验研究提供了有意义的资料。

The experiment was used Ca^2＋ import agent Ionomycin to establish intrafusal muscle cell calcium overload model of soleus muscle in rats. The confocal laser scanning microscopy was adopted to observe the effects of different concentrations of Ionomycin on resting intracelluar calcium in intrafusal fibers of rats′ soleus muscle. After administration of 2 mol/L Ionomycin, the resting intracelluar calcium in intrafusal fibers of rats′ soleus muscle increased slowly（ P 〈0.01）, and （2.67±0.43） min later after reaching the peak value, the maximum value of MFI decreased by 50%. After administration of 5 μmol/L Ionomycin, the resting intracelluar calcium in intrafusal fibers of rats′ soleus muscle increased rapidly （ P 〈0.01）, and （6.26±0.87） min later after reaching the peak value, the maximum value of MFI decreased by 50%. Ionomycin of 2 μmol/L can form a short-term calcium overload in intrafusal cells. Ionomycin of 5 μmol/L can establish a stable intrafusal muscle cell calcium overload model of soleus muscle in rats. This study provides valuable information for the study of rat muscle spindle physiology and the experimental study of calcium and weightlessness.