轮状病毒NSP5结构与功能的研究进展

Research progress in the structure and function of rotavirus NSP5

轮状病毒(RV)是世界范围内导致婴幼儿胃肠道疾病的主要病原体,RV基因组由11个分节段的双链RNA组成,分别编码6个结构蛋白(VP1、VP2、VP3、VP4、VP6和VP7)和6个非结构蛋白(NSP1、NSP2、NSP3、NSP4、NSP5和NSP6),其中第11基因片段编码非结构蛋白NSP5。NSP5蛋白较大,NSP5在病毒感染细胞中以二聚体形式存在,是一个高度磷酸化的蛋白,可与轮状病毒其他非结构蛋白或结构蛋白相互作用,具有RNA结合活性,在病毒复制过程中主要通过与NSP2的一系列相互作用发挥功能。对轮状病毒的相关结构与功能的研究十分必要,近年来,对轮状病毒的非结构蛋白进行了大量研究,主要集中在其结构、表达、功能及免疫学性质方面,现就目前国内外对轮状病毒NSP5结构与功能的研究进展进行综述。

Rotaviruses （RV） are leading causes of gastrointestinal diseases in infants and young children. The RV genome consists of 11 subunits of double-stranded RNA, encoding six structural proteins （VP1, VP2, VP3, VP4, VP6, VP7） and six non-structural proteins （NSP1, NSP2, NSP3, NSP4, NSP5, NSP6）, where the 11th gene fragment encodes the nonstructural protein NSP5. NSP5 protein is large, in the form of dimer which in the virus-infected cells, and is a highly phosphorylated protein, with rotavirus other non-structural protein or structural protein interaction, with RNA binding activity, in the virus replication process, mainly through a series of interactions with NSP2 to play a function. In recent years, a large number of non-structural proteins of rotavirus have been studied, mainly in its structure, expression, function and immunological properties, mthis study; the progress the structure and function of rotavirus NSP5 at home and abroad was reviewed.