离子色谱法测定水果中的有机酸

Determination of organic acids in fruit by ion chromatography

研究了离子交换色谱法同时测定水果中乳酸、草酸、酒石酸、柠檬酸和苹果酸含量的条件,并对分离条件进行了优化,在流速为1mL/min的0.8~30mmol/L KOH淋洗液梯度洗脱下,5种有机酸的检出限范围均为10~330μg/L。结果表明:实验考察的3种水果在贮藏期间有机酸含量先增后减,主要表现为柠檬酸的消耗,并以此判断水果的新鲜度。

An ion exchange chromatography method for simultaneous determination of lactic acid,oxalic acid ,tartaric acid,citric acid and malic acid in fruit was studied.The separation conditions were optimized,The detection limit of all five organic acids was 10-330μg/L using 0.8-30 mmol/L KOH as eluent and at a flow rate of 1 mL/min,The organic acid content of theree fruits incread first and then decreaed during storage,Tt is fesible to dertermine freshness of fruits based of cosumption of citric acid.