摘要
Objectives] This study aimed to determine the optimal medium components and culture conditions for the induction and proliferation of Dioscorea nipponica Makino axillary buds in vitro .[Methods] The effects of basic medium （MS, WPM, B5 and N6）, cytokinins type and concentration （0, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/L 6-BA; 0, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/L KT）, auxins type and concentration （0, 0.25, 0.50, 0.75, 1.00 mg/L IBA, 2,4-D and NAA） on the induction of D. nipponica axillary buds were respectively measured and compared. Then, the effects of sugar source （30 g/L sucrose, fructose, white sugar, maltose and glucose） and light intensity （0, 800, 1 600, 2 400 and 3 200 lx） on the proliferation coefficient of D. nipponica axillary buds were evaluated.[Results] Using stem segments with leaf axils as the explants, the highest induction rate （90.8%） of axillary buds was achieved in MS supplemented with 2.5 mg/L 6-BA and 0.5 mg/L 2,4-D, and the fresh and dry weights of tissue culture seedlings in this medium were also the highest, up to 1.5 g and 160.9 mg, respectively. Then, the highest proliferation coefficient of was D. nipponica axillary buds noted when sucrose was used as the sugar source in medium, and the optimal light intensity for the proliferation of D. nipponica axillary buds was 2 400 lx.[Conclusions] The results provide an experimental evidence for rapid propagation of D. nipponica .
Objectives] This study aimed to determine the optimal medium components and culture conditions for the induction and proliferation of Dioscorea nipponica Makino axillary buds in vitro .[Methods] The effects of basic medium （MS, WPM, B5 and N6）, cytokinins type and concentration （0, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/L 6-BA; 0, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/L KT）, auxins type and concentration （0, 0.25, 0.50, 0.75, 1.00 mg/L IBA, 2,4-D and NAA） on the induction of D. nipponica axillary buds were respectively measured and compared. Then, the effects of sugar source （30 g/L sucrose, fructose, white sugar, maltose and glucose） and light intensity （0, 800, 1 600, 2 400 and 3 200 lx） on the proliferation coefficient of D. nipponica axillary buds were evaluated.[Results] Using stem segments with leaf axils as the explants, the highest induction rate （90.8%） of axillary buds was achieved in MS supplemented with 2.5 mg/L 6-BA and 0.5 mg/L 2,4-D, and the fresh and dry weights of tissue culture seedlings in this medium were also the highest, up to 1.5 g and 160.9 mg, respectively. Then, the highest proliferation coefficient of was D. nipponica axillary buds noted when sucrose was used as the sugar source in medium, and the optimal light intensity for the proliferation of D. nipponica axillary buds was 2 400 lx.[Conclusions] The results provide an experimental evidence for rapid propagation of D. nipponica .
基金
Supported by University-enterprise Cooperation Project of Yanbian University([2006]3)
