反向高效液相色谱法联合紫外检测器同时测定解毒消炎茶中绿原酸等9种不同极性成分含量效果分析

Simultaneous determination of content of chlorogenic acid and other eightdifferent polarity ingredients in jieduxiaoyan tea by RP-HPLC combined with UV detector

目的:用RP-HPLC联合紫外检测器同时测定解毒消炎茶中绿原酸等9种活性成分含量。方法:采用Agilent C18(250. 0 mm×4. 6 mm,5μm)色谱柱,以乙腈(A)-0. 4%磷酸水(B)系统作为流动相,梯度洗脱(0~25 min,5%~22%A;25~31 min,22%~25%A;31~45 min,25%~45%A;45~60 min,45%~55%A;60~80 min,55%~100%A);柱温为30℃,检测波长为320 nm,流速为1. 0 m L/min。结果:绿原酸、连翘酯苷A、栀子苷、橙皮苷、连翘苷、芦荟大黄素、大黄酸、大黄素、大黄酚分别在138. 00~5520. 00 ng,30. 56~1222. 40 ng,84. 80~3392. 00 ng,108. 48~4 339. 20 ng,20. 72~828. 80 ng,20. 88~835. 20 ng,25. 76~1 030. 40 ng,33. 92~1 356. 80 ng,60. 24~2 409. 60ng范围内呈良线性关系,相关系数均大于0. 999;平均回收率均在98%~102%范围内。结论:RP-HPLC联合紫外检测器同时测定解毒消炎茶中绿原酸等9种不同极性成分灵敏、准确,分离效果较好,可为解毒消炎茶质量标准评价提供依据。

Objective：To determine the content of Chlorogenic acid and other eight active ingredients in JieduXiaoyan tea by RP-HPLC combined UV detector with simultaneous determination.Methods：The chromatographic column was Agilent C18 （250 mm×4.6 mm,5 microns） column,with acetonitrile （A） -0.4% phosphoric acid water system （B） as mobile phase,gradient elution 0～25 min,5% A～22% A;25～31 min,22%～25% A;31～45 min,25%～45% A;45～60 min,45%～55% A;60～80 min,55%～100% A）.Column temperature was 30 ℃,with detection wavelength of 320 nm and the flow rate of 1.0 mL/min.Results：Chlorogenic acid,Forsythoside A,Geniposide,hesperidin,Phillyrin,aloeemodin,rhein,emodin,chrysophanol had good linear relationship within the range of 138.00～5 520.00 ng,30.56～1 222.40 ng,84.80～3 392.00 ng, 108.48～4 339.20 ng,20.72～828.80 ng,20.88～835.20 ng,25.76～1 030.40 ng,33.92～1 356.80 ng,60.24～ 2 409.60 ng,respectively,and the correlation coefficient were greater than 0.999.The average recovery rate were all in the 98%～102% range.Conclusion：The method on Simultaneous determination of content of Chlorogenic acid and other eight eight different polarity ingredients in JieduXiaoyan tea by RP-HPLC combined UV detector have high sensitivity,high accuracy and ideal separation effect,can be used as an jieduxiaoyan tea quality evaluation.