摘要
以细菌污染的白花泡桐组培苗为材料,采用70%的酒精,0.1%HgCl_2和不同时间梯度的70%的酒精结合0.1%HgCl_23种方法来研究泡桐组培苗的脱毒技术,结果显示70%的酒精(10s)结合0.1%HgCl_2(7min)的方法效果最好,外植体的脱菌率为65.0%。
In this study, the bacterial-contaminated paulownia fortuneitissue culture seedlings were used as materials, Using 70% alcohol, 0.1% HgCl2 and 70% alcohol with different time gradient combined with 0.1% HgCl2 3 method to study the virus-free technology of Paulownia tissue culture seedlings, The results showed that 70% alcohol （ 10s ） combined with 0.1% HgCl2 （ 7 min ） was the best, and the explant removal rate was 65.0%.
作者
武文豪
李小凡
翟晓巧
Wu Wenhao;Li Xiaofan;Zhai Xiaoqiao(Institute of Paulownia,Henan Agrieultural University,Zhengzhou 450002,China;Henan Academy of Forestry,Zhengzhou 450008,China)
出处
《河南林业科技》
2018年第3期1-4,共4页
Journal of Henan Forestry Science and Technology
基金
河南省科技创新杰出创新人才计划项目
项目编号:174200510001
关键词
白花泡桐
细菌污染
脱毒
Paulownia fortunei
Bacterial contamination
Vires-free technology
