青钱柳老叶和嫩叶化学成分液质分析及5种成分含量的比较

Comparative Analysis of Chemical Constituents and Contents of Five Components in Old Leaves and Young Leaves of Cyclocandra paliurus

目的：对青钱柳老叶和嫩叶中的化学成分进行分析和鉴定,比较老嫩叶中所含成分类别及含量差异。方法：采用UPLC-Q-TOF-MS/MS分析方法,对青钱柳老叶和嫩叶50%甲醇提取液中的化学成分进行分析和鉴定。同时,采用Inter Sustain-C18色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-0.1%甲酸水溶液,流速1 m L·min-1,紫外波长254 nm,柱温30℃,梯度洗脱,对青钱柳叶50%甲醇提取液进行HPLC分析,测定其中绿原酸、山柰酚、异槲皮苷、槲皮苷、槲皮素5种成分的含量。并对结果进行主成分分析。结果：从青钱柳老叶和嫩叶中共鉴别出30种化合物,其中老叶和嫩叶共有成分21种,2种成分为嫩叶仅有,7种成分为老叶仅有。青钱柳老叶和嫩叶中5种成分含量存在差异,主成分分析将15个样品聚为老叶和嫩叶两大类。结论：青钱柳老叶和嫩叶化学成分基本相同,相对来说老叶的化学成分较嫩叶丰富,成分含量较高。建立的方法能够较好鉴别青钱柳中的化学成分,区分老叶和嫩叶,为青钱柳老嫩叶的质量控制提供依据。

Objective： To analyse and identify the chemical components between old leaves and young leaves of Cyclocandra paliurus,and compare the category and contents of components between the old leaves and young leaves. Method： The main chemical components in 50% methanol extract from old leaves and young leaves of C. paliurus were analyzed and identified by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry（ UPLC-Q-TOF-MS/MS）. The contents of chlorogenic acid, kaempferol, isoquercitrin,quercitrin and quercetin of C. paliurus were determined by high performance liquid chromatography on an Inter Sustain-C18（ 4. 6 mm × 250 mm,5 μm） with acetonitrile-0. 1% formic acid as mobile phase for gradient elution at a flow rate of 1. 0 m L·min-1,and the detection wavelength was set at 254 nm and the column temperature at30 ℃. Then the results were analyzed by using principal component analysis. Result： We finally identified 30 compounds from old leaves and young leaves. There were 21 common components among the two types of leaves. 2 components were only in young leaves, while 7 components were only in old leaves. There were quantitative differences in the contents of five components between old leaves and young leaves. 15 samples were divided into two main groups by principal component analysis. One was old leaves and the other was young leaves. Conclusion：The main chemical components in old leaves and young leaves of C. paliurus were basically similar. Comparatively,the chemical componentsin the old leaves were more abundant and the contents of five components were higher than thosein young ones. The established method could be used to betteridentify the chemical components in the leaves of C. paliurus,and distinguish the old leaves and young leaves,providing a reference for its quality control based on leaves of C. paliurus.