miR-30e和miR-223在肝癌患者中的检测及临床意义

Clinic effect of miR-30e and miR-223 in the patients with hepatocellular carcinoma

目的探讨miR-30e和miR-223在肝癌(HCC)患者中的表达水平。方法选择在我院肿瘤科就诊的不同类型的HCC患者45例为观察组,同时选取19例慢性肝病(CLD)及16例健康人为对照组(HV)。RT-qPCR检测各组血清miR-30e和miR-223的表达水平。结果 HCC患者miR-30e和miR-223血清表达水平明显低于CLD和HV组(P<0.05);同时,不同类型HCC患者血清miR-30e和miR-223均显著低于CLD和HV组表达水平(P<0.05);此外,miR-30e表达情况在HV和HCC以及CLD和HCC的AUC值分别为0.9258±0.032和0.9322±0.031,敏感性分别为89.06%和86.67%,特异性分别为81.25%和100%,cutoff值分别为0.7685和0.4755;血清miR-223表达水平在HV和HCC以及CLD和HCC的AUC值分别为1.000±0和0.9988±0.002,敏感性分别为100%和97.78%,特异性分别为100%和100%,cutoff值分别为和0.5095和0.4310;HCV-HCC患者miR-30e和miR-223在肝癌组织中均出现明显的下调(P<0.05)。结论 HCC患者血清miR-30e和miR-223水平显著降低,可作为无创早期诊断HCC疾病的指标之一。

Objective To investigate the effect of miR-30 eand miR-223 in the patients with hepatocellular carcinoma（HCC）.Methods A total of 45 HCC patients as observed group and 19 chronic liver disease（CLD）patients as well as16 healthy volunteer（HV）without any cancer as control group were studied.The expression of miR-30 eand miR-223 in the patient or healthy human were measured by RT-qPCR.Results The expression of serum miR-30 eand miR-223 were significant decreased in observed patients（P〈0.05）;Besides,serum expression level of miR-30 eand miR-223 is significantly reduced in HCC patients irrespective of etiology.Furthermore,the best cutoff value for serum miR-30 eto diagnose HCC patients and CLD was 0.7685 and 0.4755（sensitivity 89.06% and 86.67%,specificity 81.25% and100%,AUC 0.9258±0.032 and 0.9322±0.031）;The best cutoff value for serum miR-223 to diagnose HCC patients and CLD was 0.5095 and 0.4310（sensitivity 100% and 97.78%,specificity 100% and 100%,AUC 1.000±0 and0.9988±0.002）;miR-30 eand miR-223 expression levels are highly reduced in liver biopsy samples of HCC patients.Conclusion HCC were associated with down-regulation of the expression of miR-30 eand miR-223.miR-30 eand miR-223 could be novel miRNA biomarker for HCC non-invasive early diagnosis.