胆道闭锁患者术后血清尿激酶型纤溶酶原激活物受体与肝硬度值变化的关系研究

Serum urokinase plasminogen activator receptor(uPAR) is correlated with liver stiffness in postoperative biliary atresia

目的探讨血清尿激酶型纤溶酶原激活物受体(uPAR)水平与胆道闭锁(BA)患者术后肝硬度值的相关性。方法选取2014年1月-2016年12月本院择期行Kasai术的BA儿童患者48例作为研究对象(BA组)。本院同期健康体检儿童40例作为对照(正常组)。根据血清总胆红素值(TB),将BA组分成有黄疸组(n=20)和无黄疸组(n=28)。应用酶联免疫吸附法测定血清uPAR水平,应用瞬时弹性成像(TE)测量肝硬度值。结果 BA组血清uPAR水平和肝硬度值均显著高于正常组患者(P<0.001),患有黄疸的BA组血清uPAR水平和肝硬度值显著高于无黄疸组(P<0.001)。血清uPAR水平和肝硬度值及肝功能指标总胆红素(TB)、ALT、AST及碱性磷酸酶(ALP)呈正相关(P<0.001)。结论 BA患者术后血清uPAR水平与肝硬度值及肝功能密切相关,可考虑作为肝纤维化进程的非侵入性检测指标。

Objective To investigate the relationship between serum uPAR and liver stiffness in postoperative biliary atresia（BA）.Methods 48 postKasai BA children in our hospital from January 2014 to December 2016 were enrolled as study objects and 40 children from normal physical examination as control subjects.BA children were divided into two groups regarding on their serum total bilirubin（TB）：Jaundiced BA group（n=20）and Non-jaundiced BA group（n=28）.Serum uPAR was measured by enzyme-linked immunosorbent assay（ELISA）.Liver stiffness was determined using transient elastography（TE）.Results The serum uPAR level and liver stiffness scores in BA group were significantly greater than control group（P0.001）.Jaundiced BA group had substantially higher serum uPAR level and liver stiffness scores than Non-jaundiced BA group（P0.001）.Additionally,serum uPAR level was positively correlated with liver stiffness scores,total bilirubin（TB）,alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,and alkaline phosphatase（ALP）（P0.001）.Conclusion Increased serum uPAR is positively correlated with liver stiffness and liver function in postoperative BA children.Serum uPAR could be used as a noninvasive biomarker indicating the progression liver fibrosis.