一株生防地衣芽孢杆菌分泌蛋白质的鉴定及分析

Identification and Analysis of Secreted Proteins From a Biocontrol Strain of Bacillus licheniformis

以一株拮抗葡萄霜霉病的地衣芽孢杆菌生防菌为试材,采用蛋白质谱的方法对该菌在YEPD液体培养基中的分泌蛋白质进行了鉴定,研究了鉴定到蛋白质的基因本体论(GO)和京都基因与基因组百科全书(KEGG),以期为该菌株在葡萄病害生物防治中的应用提供参考依据。结果表明:分泌蛋白质中鉴定到了50种蛋白质,其相对分子量均大于10kDa。采用基因本体论对鉴定到的蛋白质进行分类,发现鉴定到的蛋白质涉及碳水化合物代谢、能量代谢、脂类代谢、氨基酸代谢和生物防御等过程,同时作为细胞组分参与核酸组成、膜结构、细胞骨架、细胞壁的组成。发现了与生物防治相关的抑菌蛋白质几丁质酶和β-1,3-葡聚糖酶,为研究地衣芽孢杆菌在葡萄病害防治中的应用提供了参考。

A selected strain of Bacillus licheniformis was used as test material,the secretory proteins of the bacterium in YEPD liquid medium were identified by the method of protein mass spectrometry.The gene ontology（GO）and the Kyoto gene and genome Encyclopedia（KEGG）were studied,in order to provide reference for the application of the strain in the biological control of grape disease.The results showed that 50 proteins were identified from the secreted proteins,and the relative molecular weight was all more than 10 kDa.By gene ontology of identified proteins were classified to the identification of proteins involved in carbohydrate metabolism,energy metabolism,and lipid metabolism,amino acid metabolism process,at the same time as the composition of cellular components involved in nucleic acids,membrane structure,cytoskeleton,and cell wall.Antibacterial proteins of chitinase andβ-1,3-glucanase related to biological control were found,which provided a reference for the application of Bacillus licheniformis in the prevention and control of grape diseases.