大岩桐SsKN1基因的克隆及组织表达分析

Cloning of SsKN1 Gene From Sinningia speciosa and Its Expression in Different Tissues

以大岩桐(Sinningia specisoa)茎尖组织为试材,采用RT-PCR方法对大岩桐SsKN1(KNOTTED1-like homeobox)基因进行克隆,经BLAST比对、邻接法构建进化树,研究了SsKN1基因与其它KNOXI基因的亲缘关系;并利用半定量RT-PCR,研究了SsKN1基因在大岩桐根、花瓣、花萼、叶、茎和茎尖等不同组织中的表达情况,以期为SsKN1基因的功能研究奠定基础。结果表明:获得了大岩桐SsKN1基因451bp的cDNA序列,该基因与烟草NTH15基因亲缘关系最近,其次为拟南芥STM基因;SsKN1基因在根、叶、茎尖和花萼等4种组织中表达,在花瓣和茎中不表达。其中在茎尖的表达量最高,叶中的表达量次之,根和花萼的表达量较低。

Sinningia speciosa were used as materials to clone SsKN1 gene via RT-PCR from shoot tips.Blast and phylogenetic trees via neighbor-joining（NJ）method were used to analysis the sequence.And then,semi-quantitative RT-PCR was used to investigate the different expression level of SsKN1 gene in different tissues of root,calyx,petal,stem,shoot tips and leaf.The results showed that 451 bp cDNA in length of SsKN1 gene was isolated,which shared a high identity with NTH15 and STM,and SsKN1 was not expressed in petal and stem while highly expressed in shoot tips and stem and relatively lower expressed in root and calyx.