摘要
前期研究中筛选到一株对大豆根腐病病原菌-尖孢镰刀菌具有较强拮抗作用的菌株Bacillus subtilis 8-32,其生防效果达32. 08%。本试验采用盆栽法,研究接种不同浓度枯草芽孢杆菌8-32(Bacillus subtilis 8-32)后,土壤酶系和微生物区系的变化情况。结果表明:使用枯草芽孢杆菌8-32后土壤脲酶活性增加了16. 9%~66. 7%,蛋白酶活性增加了88. 8%~131. 0%,酸性磷酸酶活性增加了20. 6%~51. 8%,碱性磷酸酶活性增加了7. 5%~14. 6%,根际土壤中细菌数量增加8. 5%~72. 5%,对土壤中真菌数量抑制率最高达37. 2%,放线菌数量也有明显增加。说明菌株8-32对由尖孢镰刀菌引起的大豆根腐病有显著的防治效果,具有进一步应用的潜力。
In previous study, a strain Bacillus subtilis 8-32, which had strong antagonistic ability against to Fusarium oxyspo-rum was screened, its biocontrol effect reached to 32.08%. In this experiment, further study was carried out by pot experi-ments. We studied the changing of soil enzyme system and microflora after inoculated different concentration of Bacillus subtilis 8-32. The results showed that: Bacillus subtilis 8-32 increased the activity of soil urease by 16. 9%-66.7%, protease activity increased by 88. 8% - 131.0% , acid phosphatase activity increased by 20. 6% - 51.8%, alkaline phosphatase activity in-creased by 7.5%- 14. 6% and bacteria in soil increased by 8. 5%-72. 5%, the quantity of soil fungi inhibition rate reached to 37. 2%, the number of actinomyces increased obviously. It indicated that strain 8-32 has significant control effect on soy-bean root rot caused by Fusarium oxysporum, and has potential for further application.
作者
杨可欣
王欢
刘雪娇
时向哲
贾田惠
高同国
YANG Ke-xin;WANG Huan;LIU Xue-jiao;SHI Xiang-zhe;JIA Tian-hui;GAO Tong-guo(College of Life Science, Agricultural University of Hebei, Baoding 071001, China)
出处
《大豆科学》
CAS
CSCD
北大核心
2018年第5期762-768,共7页
Soybean Science
基金
河北农业大学教改项目(2015JM04)
河北农业大学大学生创新创业训练计划(2018156)
河北省教育厅拔尖人才项目(BJ2016029)
河北省教育厅课题"生物技术"品牌特色专业建设研究项目(2012GJJG054)
河北自然科学基金青年项目(C2015204031)
教育部
河北省教育厅"生物技术"专业综合改革试点项目(201202)