人体尿液中马尿酸和甲基马尿酸固相萃取-离子色谱-电导同时测定法

Simultaneous determination of hippuric acid and methylhippuric acids in human urine by solid phase extraction-ion chromatography coupled with conductivity detector

目的建立人体尿液中马尿酸(HA)和甲基马尿酸(MHA)的离子色谱测定方法。方法采用C18固相萃取小柱萃取尿液中的HA和MHA,并用离子色谱-电导检测法检测。结果利用C18固相萃取小柱萃取尿液中的HA和MHA,去除杂质效果良好,回收率高,HA、2-MHA、3-MHA、和4-MHA 4种待测物在Metrosep A5 150阴离子交换柱(150 mm×4.0 mm i.d.,粒径5μm)中14 min之内达到基线分离,且HA和MHA的峰高与其浓度呈现良好的线性,4种待测物的检出限分别为0.02、0.02、0.02和0.04μg/ml,平均回收率为90.1%～103.8%,RSD在1.7%～4.9%之间,建立的方法用于鞋厂工人班末尿中HA和MHA的测定,均检出HA。结论该方法简单、快速、灵敏并且准确性高且绿色环保,适用于尿液中HA和MHA的测定。

[Objective]To establish an ion chromatographic method for the determination of hippuric acid（HA） and methylhippuric acids（MHA） in human urine.[Methods] HA and MHA in human urine were purified by C18 solid phase extraction column, and were determined by ion chromatography coupled with conductivity detector. [ Results ] There was a prominent effect in removing impurities and high recoveries by using C18 solid phase extraction column. HA,2-MHA,3-MHA and 4-MHA could be well separated within 14 min in an anion exchange column（150 mm×4.0 mm i.d.,5 μm particle size）,and good linear relationships between the peak height and the concentration of HA and MHA could be obtained. The detection limit of four components was 0.02,0.02,0.02 and 0.04 μg/ml,respectively. The recoveries and the RSD ranged from 90.1% to 103.8% and 1.7% to 4.9%,respectively. The developed method was applied to detect the HA and MHA in the human urine samples of shoes factory workers,and HA was detected. [Conclusion] The proposed method is sensitivity,rapid,accuracy and eco-friendly. It is suitable for the determination of HA and MHA in human urine.