老鹰茶挥发油提取工艺优化及其抑菌活性

Extraction and antibacterial activity of essential oils from hawk tea

采用水蒸气蒸馏法对老鹰茶挥发油进行提取,以挥发油得率为指标,运用单因素实验和正交实验法考察了提取时间、料液比和超声时间3个因素对老鹰茶出油率的影响,最佳提取工艺条件为:提取时间5 h,料液比1:12,超声时间60 min。验证实验表明,优选工艺条件稳定可行,在该条件下提取率为(0.29±0.03)%。运用滤纸片法评价了老鹰茶挥发油的抑菌活性,结果表明老鹰茶对大肠杆菌抑制效果最佳(11.86 mm±0.23 mm),其次是金黄色葡萄球菌、枯草杆菌及四联球菌。

To explore the influence of distillation time, solid-liquid ratio and ultrasonic time on the extraction yield of essential oils from hawk tea（Litsea coreana Levl. var. lanuginosa） using steam distillation, the single factor experiment and ortheogonal experiment were made. The optimal conditions of extraction process were distillation time of 5 h, solid-liquid ratio of 1：12 and ultrasonic time of 60 min, which was authenticated to be a high-efficiency and dependable technology with the extraction ratio of（0.29±0.03）%. And the results of antibacterial activities with disc diffusion assay suggested that the essential oils from hawk tea had the best inhibitory effect on the growth of Escherichia coli（11.86 mm±0.23 mm）, followed by Staphylococcus aureus, Bacillus subtilis and Micrococcus tetragenus.