大孔树脂吸附树莓花色苷的吸附性能

Adsorption properties of macroporous resin for adsorption of anthocyanins from raspberry

以树莓为原料,分别比较6种大孔树脂对花色苷的吸附和解吸性能,筛选出分离纯化树莓花色苷的最佳大孔树脂,在此基础上通过平衡吸附、等温吸附等方法,从动力学和热力学的角度探究大孔树脂对花色苷的吸附行为和吸附机制以及确定最佳的洗脱流速和洗脱剂浓度。研究结果表明:AB-8型大孔树脂最适合分离纯化树莓花色苷。AB-8型大孔树脂对花色苷的吸附可用准一级动力学模型描述,且吸附符合Langmuir等温吸附模型。此外,花色苷等温吸附焓变(△H_0)和吉布斯自由能变(△G_0)均小于零,说明树脂对花色苷的吸附过程属于放热、单层物理吸附,吸附可自发进行。洗脱剂浓度和洗脱流速分别在60%和3.0 mL/min时,花色苷的回收率最佳。研究结果可为AB-8大孔树脂分离纯化树莓花色苷提供理论依据。

In this study, the adsorption/desorption characteristics of raspberry anthocyanins on six of macroporous resins were compared to screen out the optimum macroporous resin for the separation and purification of raspberry anthocyanins. On this basis, the adsorption performance of macroporous resin for anthocyanin from raspberry and adsorption mechanism were investigated by equilibrium adsorption experiment, isothermal adsorption experiment, and the optimum eluant flow rate and eluant concentration were determined. The results showed that AB-8 macroporous resin was the best option for purification raspberry anthocyanins. The adsorption kinetics process of AB-8 resin for anthocyanin could well fit with Pseudo-first-order-kinetic model. The linear straight of regression displayed that the Langmuir model was more suitable. The changes of the enthalpy and the Gibbs free energy were negative. It indicated that the adsorption process of raspberry anthocyanins was exothermic and monolayer physical adsorption, and the adsorption process occurred spontaneously. The optimum elution parameters to achieve the highest recovery of anthocyanins from raspberry under an eluant concentration of 60% and elution flow rate of 3 mL/min. The results could provide a theoretical basis for the separation and purification of anthocyanins from raspberry by AB-8 macroporous resin.