内源性大麻素系统在PCOS大鼠模型子宫内膜中表达变化研究

Study of the expression of endocannabinoid system in SD rats model with PCOS

目的:研究内源性大麻素系统在PCOS大鼠模型子宫内膜中的表达变化。方法:采用来曲唑灌胃方法建立PCOS大鼠模型,观察大鼠体重、动情周期、子宫和卵巢重量变化。Real-time PCR检测内源性大麻素系统中大麻素受体1(CB1)、CB2、FAAH和大麻素合成酶(NAPEPLD)mRNA的表达变化。结果:与对照组相比,PCOS组随着给药时间的延长,体重显著性升高;21天后,PCOS组大鼠体重和卵巢重量明显升高,子宫重量明显降低;动情周期出现紊乱,均处于动情间期;CB1和NAPEPLD mRNA表达量明显升高,FAAH mRNA表达量明显降低。结论:PCOS大鼠模型子宫中内源性大麻素系统表达异常,可为今后研究内源性大麻素系统异常与PCOS的致病机理、妊娠结局关系提供理论基础。

Objective： To study the expression of endocannabinoid system in the endometrium of rats with PCOS. Methods： SD rats were fed letrozole to make rats model with PCOS. The body weight, estrus cycle, weight changes of uterus and ovarian of rats were observed. Real time PCR was used to detect the expression of endocannabinoid system, such as CB1, CB2, FAAH and NAPEPLD mRNA. Results： The prolonged time of administration of letrozole had a significant effect on increasing the weight of rats with PCOS when compared to rats in control group. After 21 days, compared with those in control group, the weight of body and ovary of rats with PCOS increased significantly, but the weight of uterus of rats with PCOS was significant lower. And the estrus cycles of rats with PCOS were disordered, the expressions of CB1 and NAPEPLD mRNA increased significantly, but the expression of FAAH mRNA decreased significantly. Conclusion： The molecular expressions of endocannabinoid system of rats with PCOS are abnormal, so it provides theoretical basis for further studies on the relationship between abnormalities of endocannabinoid system and the pathogenesis of PCOS or subsequent adverse pregnancy outcomes.