子宫内膜癌组织中miR-106b、DAB2表达与临床病理及预后的相关性

Correlation analysis of the expression of miR 106b and DAB2 in endometrial carcinoma tissue with clinical pathology and prognosis of patients with endometrial carcinoma

目的:探究子宫内膜癌组织中微小RNA106b(miR-106b)与残疾基因同源物(DAB2)表达水平与临床病理特征及患者预后的关系。方法:收集2010年5月—2015年2月本院手术切除的子宫内膜癌临床资料70例(观察组),同期本院子宫内膜癌切除手术的癌旁组织标本70例(正常组)。qRT-PCR检测子宫内膜组织中miR-106b、DAB2表达,免疫组化法检测子宫内膜组织中DAB2蛋白表达。根据miR-106b表达量分为高表达组与低表达组,观察分析miR-106b、DAB2表达与临床病理特征关系。Pearson法进行相关性分析,采用Kaplan-Meier对患者的生存情况进行分析,采用cox分析进行多因素分析。结果:观察组子宫内膜癌组织中miR-106b表达水平高于正常组,DAB2表达水平低于正常组,免疫组化结果显示观察组中DAB2蛋白阳性表达率低于正常组(均P<0.05);临床病理结果显示,miR-106b、DAB2表达均与FIGO分期、P53及Ki67显著相关,相关性分析miR-106b与DAB2表达呈负相关,KaplanMeier分析结果显示miR-106b低表达组与DAB2阳性表达组PFS均高于高表达组、阴性表达组(P<0.05);Cox多元回归分析结果显示miR-106b、DAB2表达均是患者预后的危险因素。结论:miR-106b在子宫内膜癌组织中上调表达、DAB2下调表达,二者呈负相关且均与肿瘤组织分化及细胞增殖迁移有关,可作为临床早期诊断治疗子宫内膜癌及有效评估患者预后的指标。

Objective： To investigate the relationships between the expressions of micro RNA106b（miR 106b） or DiSa- bled homolog 2 （DAB2） in endometrial carcinoma tissue and clinical pathological features and prognosis of patients with endometrial carcinoma. Methods： The clinical data of 70 patients with endometrial carcinoma who underwent sur- gical excision（ in observation group） from May 2010 to February 2015 in medical college of Nanjing university college were collected, another 70 specimens from the cancerous adjacency tissue were included in normal group at the same period. QRT PCR was used to detect the expressions of miR 106b and DAB2 in endometrial tissue, and the expression of DAB2 protein in endometrial tissue was also detected by immunohistochemistry. According to the expression level of miR 106b, the specimens were also divided into high expression group and low expression group. The relationship be- tween the expressions miR 106b and DAB2 with clinical pathological characteristics was observed and analyzed. Pearson method was used to make the correlation analysis, Kapla Meier was used to survival analysis, and multifactor analysis was carried out by Cox analysis. Results.. The expression of miR 106b in endometrial carcinoma tissue of patients in observation group was significant higher than that of patients in normal group （P〈0.05）, while the expression of DAB2 of patients in observation group was significant lower than that of patients in normal group （P〈0.05）, and the immunohistochemical results had showed that the positive rate of DAB2 protein in endometrial carcinoma tissue of patients in observation group was significant lower than that of patients in normal group （P〈0.05）. Clinical patholog ical analysis showed that the expressions of miR 106b and DAB2 were significant correlated with staging of endometrial carcinoma by FIGO, and level of P53 and Ki67 （P〈 0.05）. Pearson correlation analysis had showed that there was negative correlation between miR 106b expression and DAB2 expression （P 〈 0.05）. Kaplan Meier analysis had showed that the level of PFS in miR 106b low expression group was significant higher than that in miR 106b high ex- pression group （P 〈 0.05）, and the level of PFS in DAB2 positive expression group was also significant higher than that in DAB2 negative expression group （P 〈 0.05）. Cox multiple regression analysis had showed that the expressions of miR 106b and DAB2 were risk factors for adverse prognosis of patients. Conclusion： In endometrial carcinoma tissue of patients, the expression of MiR 106b increases, but the expression of DAB2 decreases, which are negatively correla- ted between the expression of MiR 106b and DAB2. And the expression of MiR 106b and DAB2 are all related to tumor tissue differentiation, and tumor cell proliferation and migration, so it can be used as an indicator for early diagnosis and evaluation of prognosis of patients with endometrial carcinoma.