整合素α2β1和CD44V3表达与宫颈鳞癌临床病理关系探究

Relationship between the expression of integrin α2β1 or CD44V3 and the clinicopathology of cervical squamous cell carcinoma

目的:分析整合素α2β1和CD44V3在宫颈鳞癌中的表达及临床意义。方法:收集2015年1月—2017年1月本院诊治的宫颈鳞癌标本36例(宫颈鳞癌组)、子宫平滑肌瘤手术切除的良性病变宫颈黏膜组织标本36例(对照组),应用免疫组化检测检测宫颈组织中的整合素α2β1、CD44V3的mRNA表达水平。结果:对照组整合素α2β1mRNA的表达值(0.09±0.03)低于宫颈鳞癌组(0.52±0.08)(t=30.197,P=0.000),整合素CD44V3mRNA的表达(0.13±0.02)低于宫颈鳞癌组(0.65±0.11)(t=27.906,P=0.000);整合素α2β1和CD44V3在宫颈鳞癌组织中阳性率(66.7%、72.2%)均高于对照组(27.8%、38.9%)(χ~2=10.923、8.100,P=0.001、0.004);整合素α2β1和CD44V3的阳性表达与宫颈鳞癌患者的年龄无相关性(P>0.05),但与宫颈鳞癌患者的PTNM分期、分化程度、是否淋巴结转移及浸润深度均存在相关性(P均<0.05)。整合素α2β1和CD44V3在宫颈鳞癌组织中的表达呈正相关性(r=0.369,P<0.05)。结论:整合素α2β1和CD44V3在宫颈鳞癌组织中呈高表达,且与患者的PTNM分期、分化程度、是否淋巴结转移及浸润深度密切相关,整合素α2β1和CD44V3联合检测可为宫颈鳞癌患者的诊治及预后评估提供参考。

Objective： To analyze the expression of integrin α2β1 and CD44V3 in cervical squamous cell carcinoma, and to explore its clinical significance. Methods： Cervix tissues specimens of 72 women were collected from Tianjin gyne- cology and obstetrics central hospital, the third hospital of Hebei medical university, and Hebei provincial traditional Chinese medicine hospital from 2015 to 2017, which included 36 cases with cervical squamous cell carcinoma （in obser- vation group） and 36 cases with uterine leiomyoma （in control group）. The expressions of integrin α2β1 and CD44V3 in cervix tissues were detected by immunohistochemistry （SP method）. Results： The expression of integrin α2β1 mRNA in cervix tissues（0.09±0.03） of women in control group was significant lower than that （0.52±0.08） of women in ob servation group （t =30.20, P =0.000）. The expression of integrin CD44V3 mRNA of women in control group （0.13±0.02） was significant lower than that（0.65±0.11 ） of women in observation group （t=27.91, P=0.000）. The positive rates of integrin α2β1 and CD44V3 in cervix tissues of women in observation group were 66.7% and 72.2%, respective ly, which were significantly higher than those of women （27.8% and 38. 9%, respectively） in control group （X^2 = 10.92, 8.10, P = 0.001, 0.004）. There was no significant correlation between the expression of integrin α2β1 or CD44V3 and the age of women with cervical squamous cell carcinoma （P〉0.05）, but there were significant correlation between the expression of integrin a2131 or CD44V3 and the PTNM staging, differentiation degree of carcinoma cell , lymph node metastasis and depth of invasion （all P（0.05）. The expression of integrin α2β1 and CD44V3 in cervical squamous ceil carcinoma had positively correlated （r=0.369,P〈0.05）. Conclusion： The expressions of integrin α2β1 land CD44V3 in cervix tissues of women with cervical squamous cell carcinoma are high, which are closely related to the PTNM staging, differentiation degree of carcinoma cell, lymph node metastasis and depth of invasion. The combined detection of integrin α2β1 and CD44V3 can be used as reference for diagnosis and prognosis evaluation of cervical squa- mous cell carcinoma.