夏大豆重组自交系群体籽粒蛋白质含量QTL定位

QTL Mapping of Grain Protein Content in Recombinant Inbred Lines of Summer Soybean

大豆籽粒蛋白质含量是复杂数量性状,目前对中国夏播大豆籽粒蛋白质含量等品质性状遗传基础的了解相对较少。本研究对以江淮地区夏大豆蒙8108与骨干亲本南农1138-2杂交育成的NJMN重组自交系群体进行了5个环境田间试验获得表型数据,利用含2 062个SLAF标记的遗传图谱对大豆籽粒蛋白质含量进行加性、上位性QTL定位。结果发现NJMN群体籽粒蛋白质含量存在超亲分离,不同种植环境、家系与环境间互作均存在显著差异。在6号、7号、11号、17号染色体上定位到4个控制籽粒蛋白质含量的加性QTL,其中qProt-17-1未见前人报道,其与环境间存在显著互作效应。还发现3对加性×加性上位QTL,其总的效应值和表型贡献率均高于加性QTL,表明非加性效应在NJMN群体蛋白质含量遗传体系中起了重要作用。

Soybean grain protein content is a complex quantitative trait, and and the genetic basis of quality traits such as the grain protein content of Chinese summer soybean are relatively less understood. Five environmental field experiments were conducted to obtain phenotypic data of NJMN recombinant inbred line population, which was bred by crossing summer soybean Meng8108 with Nannong1138-2, a key parent in Jianghuai area. Additive and epistatic QTL location for seed protein content was applied by using the genetic map with 2 062 specific-locus amplified fragment markers. The results indicated that there was an obvious transgressive segregation of seed protein content in NJMN population grains, and significant differences were existed in different planting environments, families and the interactions among environments. Four additive QTLs for controlling grain protein content were mapped on chromosome 6, 7, 11 and 17. Among them, qProt-17-1 had not been reported previously and had significant interaction with environment. Three pairs of additive × additive epistatic QTL were found, and the total effect valueand phenotypic contribution rate were higher than those of the four additive QTL. It indicated that the non-additive effect might play an important role in the genetic base for seed protein content in NJMN population.