大豆异黄酮对慢性脑缺血大鼠海马胆碱能神经元的保护作用

PROTECTIVE EFFECTS OF SOYBEAN ISOFLAVONE ON HIPPOCAMPAL CHOLINERGIC NEURONS OF RATS DURING CHRONIC CEREBRAL ISCHEMIA

目的:研究大豆异黄酮对慢性脑缺血大鼠海马胆碱能神经元的保护作用。方法:健康成年雌性SD大鼠30只,随机分为假手术组、模型组、治疗组(n=10),模型组、治疗组采用永久结扎去势大鼠双侧颈总动脉的方法建立慢性脑缺血大鼠模型,假手术组分离双侧卵巢和颈总动脉,不结扎。建立模型后,治疗组术大鼠给予大豆异黄酮(9mg/kg/d)灌胃21d。采用SP免疫组织化学染色法观察各组大鼠海马CA1区神经元胆碱乙酰基转移酶(cholineacetyltransferase,ChAT)蛋白表达的变化。采用MiVnt图像分析系统对免疫组织化学染色阳性结果进行定量分析。结果:假手术组大鼠海马CA1区ChAT蛋白的表达显著多于模型组和治疗组(P<0.05);治疗组大鼠海马CA1区ChAT蛋白的表达较模型组明显增多(P<0.05)。结论:大豆异黄酮对慢性脑缺血大鼠海马胆碱能神经元具有保护作用,其机制可能与上调海马神经元ChAT蛋白的表达有关。

Objective： To investigate the protective effects of soybean isofavone on hippocampal cholinergic neurons of chronic cerebral ischemia rats. Methods： 30 healthy, adult female SD rats were applied in this study and randomly divided into sham operation group （n=10）, model group （n=10） and treatment group （n=10）. The rats in model group and treatment group were established chronic cerebral ischemia model by permanently ligating the common carotid arteries on both sides of castrated rats. In sham operation group, the bilateral ovaries and common carotid arteries were only separated without ligature. After the chronic cerebral ischemia rats’ model was established, the rats in treatment group were lavaged with soybean isofavone （9mg/kg/d） for 21 days. SP immunohistochemical staining and MiVnt image analysis system were used to detect and analyze the choline acetyltransferase （ChAT） protein expression in CA1 region of hippocampus of rats in each group. Results： The ChAT protein expression of neurons in CA1 region of hippocampus of rats in sham operation group was obvioulsy higher than model group and treatment group （ P〈0.05）. The ChAT protein expression of neurons in CA1 region of hippocampus of rats in treatment group was obvioulsy higher than model group （ P〈0.05）. Conclusions： Soybean isofavone has protective effects on cholinergic neurons in hippocampus of rats with chronic cerebral ischemia, the mechanisms may be related to soybean isofavone can up regulate ChAT protein expression in hippocampus.