花多多对金线莲组培苗和移栽成活率的影响

Effects of Adding “Huaduoduo” Fertilizer into Culture Medium on Growth Potential and Transplanted Survival Rate of Anoectochilus roxburghii Plantlets

以金线莲品种"泰宁一号"为试验材料,用MS与B5作为基础培养基,添加不同种类不同用量的花多多肥料,筛选出有利于金线莲组培苗培育壮苗和提高移栽成活率的最优培养基。结果表明:不同组合培养基对金线莲根茎和叶生长影响存在着明显差异,MS+添加物更有利于根、茎生长;而B5+添加物更有利于叶片生长;同时,与空白MS和B5培养基相比,适当增添不同量花多多可以显著地改善金线莲组培苗的长势、增加内含物和提高移栽成活率。其中,尤以MS+2 g花多多1号的处理更利于培育壮苗、提高移栽成活率。

The Anoectochilus roxburghii cultivar “Taining No. 1” was used as experimental material, and the optimum medium for cultivating the strong plantlets and enhancing the transplanted survival rate of plantlets was screened out by adding differentkinds and levels of “Huaduoduo” fertilizer into MS and B5 basic medium. The results showed that the effects of different combinations of media on the growth of root, stem and leaf of A. roxburghii plantlets were significantly different, and MS＋ additives wasmore conducive to the growth of root and stem, while B5＋ additives was more conducive to leaf growth. In comparison with theblank MS and blank B5 without additives, the MS or B5 basic culture medium with appropriate quantity of “Huaduoduo” fertilizercould significantly improve the growth vigor of A. roxburghii plantlets, increase their inclusions, and improve their survival rate after transplantation. Among all treatments, the treatment MS＋ 2 g “Huaduoduo No. 1” was more beneficial to the cultivation ofstrong plantlets and the improvement of their transplanted survival rate.