犬子宫蓄脓中绿脓杆菌分离鉴定及耐药性分析

Isolation, identification and susceptibility analysis of Bacillus pyocyaners from a canine pyometra

为调查伴侣动物化脓性疾病的致病原因,对来天津农学院附属动物医院就诊的11岁龄母犬蓄脓子宫脓汁进行病原菌的分离、鉴定和耐药性分析。根据分离菌菌落形态观察、涂片染色镜检、生理生化试验、16S rRNA基因序列分析鉴定结果表明,该分离菌为绿脓杆菌;药敏试验结果显示该分离菌对氨曲南、头孢呱酮、头孢噻肟、头孢吡肟、头孢他啶、氧氟沙星、链霉素、卡那霉素、呱啦西林和妥布霉素高度敏感,对头孢曲松、头孢呋辛、头孢西叮中度敏感,对氨苄西林、头孢唑林、头孢唑吩、麦迪霉素耐药。研究结果为防治由绿脓杆菌引起的相关感染提供参考依据。

In order to clarify the causes of the fester disease in companion animals, the study involved isolation, identificationand drug susceptibility analysis of pathogenic bacteria from an 11-year-old female dog’s uterus who were admitted to theveterinary hospital of Tianjin Agricultural University. According to the results of colony morphology, smear staining microscopy,physiological and biochemical reaction and 16S rRNA gene analysis, the bacteria was identified as a Bacillus pyocyaners.Susceptibility analysis showed that the isolate is highly sensitive to aztreonam, cefdinir, cefotaxime, cefepime, ceftazidime,ofloxacin, streptomycin, kanamycin, piperacillin and tobramycin. Moreover, this isolate is moderately sensitive to ceftriaxone,cefuroxime and cefoxitin, but resistant to ampicillin, cefazolin, cefazine and medemycin. The research results providedreferences for controlling infection caused byBacillus pyocyaners.