长非编码RNA H19在结直肠癌化疗敏感性中的作用研究

Role of long noncoding RNA H19 in chemosensitivity of colorectal cancer

目的研究长非编码RNA H19在结直肠癌化疗药物敏感性中作用。方法分别构建lncRNA H19稳定过表达及表达沉默的结肠癌细胞株,分别加入5-FU培养,RT-qPCR检测H19表达,MTT法检测各组细胞增殖率,通过划痕实验检测各组迁移能力;Western blot检测各组细胞凋亡蛋白、耐药蛋白MDR1、MRP1和BCRP表达。结果与正常细胞比较,lncRNA H19在结直肠癌细胞中表达升高,lncRNA H19稳定过表达组细胞增殖率明显高于H19表达沉默组,lncRNA H19稳定过表达组细胞48 h迁移距离明显大于H19表达沉默组,lncRNA H19稳定过表达组Bax蛋白表达下调,BCL-2、MDR1、MRP1和BCRP表达上调,H19表达沉默组Bax蛋白表达上调,BCL-2、MDR1、MRP1和BCRP表达下调。结论 lncRNA H19高表达的结直肠癌降低结直肠癌对化疗药物的敏感性,下调lncRNA H19表达可提高化疗药物的疗效。

Objective To study the role of long non-coding RNA H19 in the chemosensitivity of colorectal cancer. Methods The colon cancer cell lines stably overexpressed and silenced with lncRNA H19 were constructed and cultured in 5-FU. The expression of H19 was detected by RT-qPCR. The cell proliferation rate was measured by MTT assay. The migration ability of each group was evaluated by scratch test. The protein expressions of MDR1, MRP1 and BCRP were detected by Western blot. Results Compared with normal cells, the expression of hicRNA H19 increased, and the proliferation rate of lncRNA H19 stable overexpression group was significantly higher than that of H19 silencing group. The migration distance in lncRNA H19 stable overexpression group was significantly longer than that in H19 silencing group and the overexpression of bcl-2 protein was down-regulated and bax, MDR1, MRP1 and BCRP was up-regulated in lncRNA H19 stable overexpression group. The expression of bcl-2 protein was up-regulated and the expression of bax, MDR1, MRP1 and BCRP was down-regulated in H19 silencing group. Conclusion Colorectal cancer with high expression of lncRNA H19 reduces the sensitivity of colorectal cancer to chemotherapeutic drugs, and down-regulation of lncRNA H19 expression can improve the curative effect of chemotherapy drugs.