苦参碱衍生物的合成及其对人胃癌SGC-7901细胞增殖抑制的影响

Synthesis of matrine derivative and inhibitive effect of derivative on the proliferation of human gastric cancer SGC-7901 cells

目的:合成一种新的苦参碱衍生物,并研究此衍生物对人胃癌SGC-7901细胞增殖抑制的影响。方法:以苦参碱为原料,水解后与氢化钠,芳香醛类化合物反应得到终产物。以苦参碱做对照,不同浓度的衍生物处理人胃癌SGC-7901细胞,通过MTT法观察其对癌细胞增殖的影响。结果:合成一个未见文献报道的苦参碱衍生物,并对其进行了结构表征。该衍生物在浓度大于200μM时对人胃癌SGC-7901细胞呈现明显增殖抑制作用。结论:合成的苦参碱衍生物在体外具有较苦参碱更强的细胞增殖抑制作用。

Objective：A novel matrine derivatives was synthesized and its effects on the proliferation of human gastric cancer SGC-7901 cells was evaluated. Methods：The final product was prepared by a three-step reaction of hydrolysis,esterification and condensation from Matrine. Human gastric cancer SGC-7901 cells were cultured and inhibitive effects of different concentration on SGC-7901 cells proliferation were evaluated by MTT assay. Results：A novel matrine derivatives was obtained and its structure was characterized. The experiments in vitro showed that the inhibitive effect of matrine on the proliferation of human gastric cancer SGC-7901 cells was significant at the concentration of more than 800 μM. Conclusion：The inhibitive effects of derivative on human gastric cancer SGC-7901 cells proliferation was better than that of matrine in vitro.