高效液相色谱法测定冬凌草甲素脂质体的含量

Determination of oridonin in oridonin Liposome by HPLC

目的:建立高效液相色谱法(HPLC)测定冬凌草甲素脂质体的含量。方法:色谱柱:Agilent Zorbax C_(18)柱(250 mm×4.6 mm,5μm),流动相:甲醇-水(55∶45),检测波长:241 nm,柱温:30℃,流速:1 mL/min;进样量:20μL。结果:冬凌草甲素在10~100μg/mL之间线性关系良好,r=0.999 5。日内、日间RSD为0.8%和1.2%,重复性RSD为1.92%,方法回收率平均为99.9%,RSD为0.77%,样品的平均包封率为95.0%。结论:该法简便,准确度高,可用于冬凌草甲素脂质体的含量测定。

Objective：A high performance liquid chromatography（HPLC）method was established for the determination of oridonin in ozagrel hydrochloride liposome. Methods：A C（18） column（250 mm ×4.6 mm,5 μm）was used with the mobile phase of methanol-H2O（55 ∶45）at the detection wavelength of 241 nm. The flow rate was at 1.0 mL/min. The column temperature was 30 ℃. 20 μL sample was injected. Result：The linear range of oridonin was 10~100 μg/mL（r=0.999 5）.The inter-and intra-day RSD was 0.8%,1.2%. The method recoveries were 99.9%. The average entrapment efficiency of tartrate indoquinoline liposome was 99.9%（RSD=0.77%）. Conclusion：This method is simple,accurate and can be used to control the quality of oridonin Liposome.