2016-2017年度鹤壁市流感病毒的快速检测分析

Analysis of influenza virus rapid detection in Hebi in 2016-2017

目的通过对流感病毒的检测分析,掌握鹤壁市当时的流感病毒。方法对2016-2017流行年度流感样病例的咽拭子样本,采用双通道real-time RT-PCRR方法检测流感病毒特异性核酸,先检测A型和B型通用引物,再对A型阳性样本进行亚型H1N1和H3N2的检测。另选部分样本进行重复性试验,同时用普通realtime RT-PCR(单通)进行比对复核,以检验结果的准确性。结果 678份咽拭子样本中检出130份流感病毒核酸呈阳性,其中3份为甲型H1N1,127份为甲型H3N2。10份样本的双通道real-time RT-PCR方法的重复性试验和与普通real-time RT-PCR方法的比对试验结果一致。结论鹤壁市的流感病原为甲型H1N1、H3N2,以H3N2为主。双通道real-time RT-PCR技术可用于流感病毒甲型H1N1和H3N2的实验室快速诊断。

Objective To understand the current influenza virus in Hebi through the detection and analysis of influenza virus. Methods 2016-2017 annual influenza-like cases of pharyngeal swabs were regarded as samples, and virus specific nucleic acids were detected by the dual channel real time RT-PCRR method. Type A and type B were tested firstly, and then type A subtype H1 N1 and H3 N2 test positive samples. Using an alternate partial as repeatable test samples, at the same time using ordinary real-time RT-PCR（single） for review, to check up the accuracy of the results. Results 130 influenza virus nucleic acids were detected positive from 678 throat swabs, 3 of which were A/H1 N1 and 127 were A/H3 N2. 10 samples of dual channel real-time RT-PCR method repeatability test had the same test results with ordinary real-time RT-PCR method. Conclusion The influenza pathogens in Hebi city are type A H1 N1 and H3 N2, mainly H3 N2. Dual channel real-time RT-PCR technology can be used for rapid laboratory diagnosis of influenza A/H1 N1 and A/H3 N2.