组织工程化人口腔黏膜移植对裸鼠创面修复作用研究

Study on the effect of grafted tissue-engineered human oral mucosa on the wound healing in nude mice

目的评价组织工程化人口腔黏膜移植对裸鼠创面的修复作用。方法该研究于2016年11—12月在中国医学科学院基础医学研究所实验动物中心完成。随机选取健康雄性裸鼠8只(3～4周龄,体重为14～15 g),用0.8%戊巴比妥钠麻醉,在其颈部及背部各制备大小约1.0 cm×1.0 cm的缺损,颈部缺损用组织工程化人口腔黏膜移植修复,背部缺损用真皮基质(acellular dermis matrix,ADM)移植修复。饲养8 d后取下移植皮片,HE染色观察组织特点。结果 HE染色发现,组织工程化人口腔黏膜移植物形成类皮肤组织结构,但部分上皮细胞脱离生物支架及ADM开始降解;而ADM移植物表面未见上皮细胞爬行。结论组织工程化人口腔黏膜移植能促进裸鼠创面修复。

Objective To investigate the role of grafted tissue-engineered human oral mucosa in the wound healing of nude mice. Methods This study was conducted at the Animal Experimental Center of Institute of Basic Medicine of Chinese Academy of Medical Sciences from November 2016 to December 2016. Select 8 healthy nude mices,male,aged3 to 4 weeks,weighing 14 ~ 15 g. Animals were anesthetized with 0.8% pentobarbital sodium. A 1.0 cm×1.0 cm fullthickness skin wound was created in the neck and back region in every mice,leaving the fascia intact. Tissue-engineered human oral mucosa was placed on the fascia of the neck and fixed in place with suture,and then fixed with a bolster dressing. In the control group,acellular dermis matrix（ADM）was placed on the fascia of the back,and all other surgical manipulation was the same as in the tissue-engineered human oral mucosa group. Eight days after grafting with the tissue-engineered human oral mucosa and ADM,the bolster dressings were removed. Histopathologic examination of the skin flaps was performed by HE staining. Results Histopathological analysis of tissue-engineered human oral mucosa graft revealed a well-developed,multilayered and stratified squamous epithelium,and resembled native skin. But a portion of the tissue-engineered human oral mucosa epithelial layer peeled away from the ADM and ADM began to degrade. In contrast,no epithelial coverage of the ADM observed for 8 days after grafting. Conclusion Wound healing can be enhanced by using tissue-engineered human oral mucosa graft.