香附四物汤挥发油β-环糊精包合物的制备、表征与成分分析

Inclusion Complex of XFSWO/β-Cyclodextrin: Study on Its Preparation,Characterization and Component Analysis

目的:采用环糊精包合技术将香附四物汤中具有显著活性的挥发油部位制成β-环糊精包合物,以解决香附四物汤新型口服制剂研发过程中挥发油存在的挥发性与不稳定性等问题。方法:采用正交试验确定饱和水溶液法制备香附四物汤挥发油(XFSWO)/β-环糊精(β-CD)包合物的最佳包合工艺为XFSWO:β-CD 1:10 (m L:g),包合温度40℃,包合时间1 h;通过差示扫描量热法(DSC)、红外光谱法(IR)、X射线衍射法(XRD)、扫描电镜法(SEM)对制得的包合物进行表征,证明包合成功;采用气相色谱-质谱联用法(GC-MS)进行XFSWO及XFSWO/β-CD包合物的成分分析与比较。结果:β-CD包合未对XFSWO中主要成分的化学性质产生影响,但组分含量可能发生一定改变。结论:本研究为香附四物汤新型口服制剂的研发提供了理论依据,为后续的研究工作奠定了基础。

Objective ： Essential oil of Xiangfu Siwu Decoction （XFSWO） was prepared into β - cyclodextrin （β - CD） bycyclodextrin camplexation techniques, thus to solve the volatility and instability of the essential oil in the new oral prepa-ration of Xiangfu Siwu Decoction. Methods ：The optimizing saturated solution method for XFSWO/β - CD was determined with orthogonal experiment. The optimal process was as follows： XFSWO ：β -CD was 1：10 （mL：g） , temperature was aws at 40℃, clathration time was 1 h. The results of differential scanning calorimetry（ DSC）, infrared spectrometry（ IR）, X -ray diffraction（XRD） and scanning electron microscopy（SEM） demonstrated the clathration of XFSWO/β -CD wassuccessful. Results ：The major components of XFSWO and XFSWO/β -CD were analyzed and compared with gas chro-matography- mass spectrography （GC -MS）. GC -MS results revealed that there was no change in the components,but a slight change in the contents of some components. Conclusion ：This study provided theoretical basis for the new oralpreparation of Xiangfu Siwu Decoction, and laid the foundation for the further study.