子宫内膜异位症患者在位和异位内膜组织中ER和TrkB的表达及发病机制

The expression and pathogenesis of estrogen receptor and tyrosine kinase receptor B in the eutopic and ectopic endometrium tissues of patients with endometriosis

目的:探讨子宫内膜异位症(EMs)患者在位和异位内膜组织中雌激素受体(ER)和酪氨酸激酶受体B(TrkB)的表达及发病机制。方法:选取2017年7月至2017年12月收治的52例子宫内膜异位症患者,患者均行腹腔镜下盆腔子宫内膜异位病灶清除术,其中内膜增殖期、分泌期各26例。术中留取异位、在位内膜组织52例,均经病理学证实为子宫内膜异位症。采用免疫组织化学法、实时聚合酶链反应、蛋白印迹法检测在位、异位内组织的ERα、ERβ、TrkB mRNA和蛋白质的表达差异。结果:EMs患者异位内膜组织ERβ、TrkB mRNA表达高于在位内膜组织,而其ERαmRNA表达水平低于在位内膜组织(P <0. 05); EMs异位内膜ERβ、TrkB蛋白表达高于在位内膜(P <0. 05),而其ERαmRNA表达水平低于在位内膜;在位EMs增殖期内膜ERα、ERβ、TrkB蛋白表达高于分泌期内膜(P <0. 05)。结论:ERβ和TrkB可能参与子宫内膜异位症的发病过程。

Objective： To investigate the expression and pathogenesis of estrogen receptor（ ER） and tyrosine kinase receptor B（ TrkB） in eutopic and ectopic endometrium of patients with endometriosis（ EMs）. Methods： A total of 52 patients with endometriosis admitted from July 2017 to December 2017 were selected. All patients underwent laparoscopic pelvic endometriosis removal,including 26 cases of endometrial proliferative phase and 26 cases of secretory phase. 52 cases of ectopic and eutopic endometrium were retained during the operation,all of which were confirmed by pathology as endometriosis. Immunohistochemical method,real-time polymerase chain reaction and Western blot were used to detect the expression differences of ERα、ERβ、TrkB mRNA and protein in eutopic and ectopic tissues. Results： The expression of ERβ and TrkB mRNA in ectopic endometrium of EMs patients was higher than that in eutopic endometrium,while the expression level of ERα mRNA was lower than that in eutopic endometrium tissues（ P 〈 0. 05）. The expression of ERβ and TrkB protein in the ectopic endometrium of EMs was higher than that of the eutopic endometrium,but the expression level of ERα mRNA was lower than that of the eutopic endometrium,and the difference was statistically significant（ P 〈 0. 05）. The expression of ERα,ERβ and TrkB protein in the eutopic EMs proliferative phase was higher than that of the secretory endometrium,and the difference was statistically significant（ P 〈 0. 05）. Conclusion： Estrogen receptor β and tyrosine kinase receptor B may be involved in the pathogenesis of endometriosis.