G蛋白偶联受体81、单羧酸转运体1和4在宫颈鳞癌中的表达及其临床意义

Expressions of GPR81, MCT1 and MCT4 in squamous carcinoma and their clinical significance

目的:通过检测G蛋白偶联受体81(G protein-coupled receptor 81,GPR81)、单羧酸转运体(monocarboxylate transporter,MCT)1和4在宫颈鳞癌中的表达,探讨GPR81,MCT1,MCT4在宫颈鳞癌发病中的作用。方法:采用免疫组织化学法(SP二步法)检测GPR81,MCT1,MCT4在16例正常宫颈组织、44例宫颈鳞癌组织中的表达情况,分析其表达水平与宫颈鳞癌临床分期和病理的关系,研究GPR81,MCT1,MCT4在宫颈鳞癌组织中表达的相关性。结果:宫颈鳞癌组中的GPR81,MCT1,MCT4表达水平均明显高于正常宫颈组(P<0.01)。在宫颈鳞癌组中,临床分期为I^II期宫颈鳞癌组织中的GPR81,MCT1,MCT4表达低于临床分期为III^IV期者,其中GPR81的表达差异有统计学意义(P<0.05),MCT1和MCT4的表达差异无统计学意义(P>0.05);中分化肿瘤组的GPR81,MCT1,MCT4表达低于低分化肿瘤组,但差异无统计学意义(P>0.05);无淋巴结转移的宫颈鳞癌组与有淋巴结转移的宫颈鳞癌组的GPR81,MCT1,MCT4表达无明显差异(P>0.05)。GPR81,MCT1,MCT4在宫颈鳞癌组织中的表达无明显相关关系(P>0.05)。结论:GPR81,MCT1,MCT4可能与宫颈鳞癌的发病有关,GPR81可能与宫颈鳞癌的病情进展有关。

Objective： To validate the expressions of G protein-coupled receptor 81 （GPR81）, monocarboxylate transporter （MCT） 1 and MCT4 in cervical squamous carcinoma and to explore their role in the onset of cervical squamous carcinoma. Methods： Immunohistochemical method was used to detect the expressions of GPR81, MCT1 and MCT4 in 16 normal cervical tissue and 44 cervical squamous carcinoma tissue. The associations of these proteins expression with cervical squamous carcinoma or clinicopathological factors were analyzed. Results： The expressions of GPR81, MCT1 and MCT4 in cervical squamous carcinoma tissue were higher than those in normal cervical tissue （P〈0.01）. In cervical squamous carcinoma group, the expressions of GPR81, MCT1 and MCT4 in clinical stage Ⅰ–Ⅱ group were lower than those in clinical stage Ⅲ–Ⅳ group, with significant difference （P〈0.05）. The expressions of GPR81, MCT1 and MCT4 in middle-differentiated tumor group were lower than those in low-differentiated tumor group, but there was no significant difference between the two groups （P〉0.05）. No difference of the expressions of GPR81, MCT1 and MCT4 were found between cases with or without lymphatic metastasis （P〉0.05）. No correlation was found among GPR81, MCT1 and MCT4 in cervical squamous carcinoma （P〉0.05）. Conclusion： GPR81, MCT1 and MCT4 may be associated with the onset of cervical squamous carcinoma, and GPR81 may be associated with the progression of cervical squamous carcinoma.