环状RNA circ_001988在结直肠癌中的表达及其临床意义

The expression of circ_001988 in colorectal cancer and its clinical significance

目的探讨患者环状RNA circ_001988在结直肠癌患者中的表达及其与临床病理特征及预后的关系。方法实时荧光定量聚合酶链反应（qRT—PCR）法检测结直肠癌及癌旁正常组织中circ_001988的表达量。分析circ_001988的表达差异与临床资料及病例特征的关系及circ_001988的表达差异与临床预后的相关性。采用Cox比例风险回归模型进行单变量和多变量回归分析。结果circ_001988在结直肠癌组织中的相对表达水平低于癌旁正常组织（P〈0．01）。circ_001988低表达与与淋巴结转移（P〈0．05）、远处转移（P〈0．05）和TNM分期（P〈0．05）密切相关。生存曲线（Kaplan～Meier）分析显示，circ_001988高表达患者与circ_001988低表达患者相比，总体存活率显著较好（P〈0．01）。单变量Cox比例风险回归模型分析显示，circ_001988表达水平、淋巴结转移、远处转移和TNM分期与结肠癌患者的总生存率相关（P〈均0．05，）。多变量分析确定circ_001988为结直肠癌独立预后因素。结论circ_001988低表达与结直肠癌转移和预后相关。因此，circ_001988可以作为预测结肠癌患者预后的潜在生物标志物。

Objective To investigate the expression of circ_001988 in colorectal cancer patients and to discuss its correlation with the clinicopathological features and prognosis of eolorectal cancer. Methods qRT - PCR was performed to measure the expression levels of circ_001988 in coloreetal cancer tissues. The expression of eirc 001988 was assessed with respect to clinicopathological parameters. Kaplan - Meier analysis was performed to identify survival differences in colorectal cancer patients. Univariate and multivariate analysis were performed using the Cox proportional hazard analy- sis. Results circ_001988 was significantly down - regulated in colorectal cancer tissues compared to normal tissue sam- ples （P 〈 0.01 ）, and a low expression of circ_001988 was found to be significantly associated with lymph node metasta- sis （P 〈0.05）, distant metastasis （P 〈0.05） and TNM stage （P 〈0.05）. Moreover, Kaplan - Meier survival analysis revealed that high circ_001988 expression predicted significantly better overall survival （P 〈 0.01 ）. In addition, univari- ate Cox proportional hazards regression model analysis indicated that relative circ_001988 expression level, lymph node metastasis, distant metastasis and TNM stage were correlated with an overall survival rate in colorectal cancer patients. Finally, multivariate analysis results indicated that circ_001988 was an independent prognostic factor in colorectal cancer. Conclusions Reduced circ -001988 expression contributes to colorectal cancer metastasis and poor prognosis. Thus, circ_001988 might serve as a promising biomarker for prognosis of colorectal cancer.