徐州地区汉族人群CYP1B1和GSTP1基因多态性与肺癌易感性的相关性研究

Correlation between CYP1B1 and GSTP1 genetic polymorphisms and susceptibility to lung cancer among Han population in Xuzhou

目的探讨徐州地区汉族人群CYPIBl基因rs1056836位点，GSTPl基因rs1695位点单核苷酸多态性与肺癌易感性的关系。方法采用病例对照研究方法，收集徐州市肿瘤医院病理确诊的肺癌患者100例作为实验组；选取同期徐州市肿瘤医院体检中心正常人群100例作为对照组。采用Taqmanreal—time PCR方法对CYP1B1基因rs1056836位点、GSTP1基因rs1695位点单核苷酸多态性进行基因分型，并按吸烟状态分层分析两位点单核苷酸多态性与肺癌的易感性。结果携带CYP1B1基因rs1056836位点和GSTPI基因rs1695位点突变杂合型和纯合型的个体患肺癌的风险均升高（OR=4．16，3．77和0R=2．90，3．04），CYP1B1基因rs1056836位点和GSTP1基因rs1695位点单核苷酸多态性在增加肺癌风险上有协同作用（OR=11．67，95％CI 2．41—56．48，P〈0．01）；CYP1B1基因rs1056836位点和GSTP1基因rs1695位点携带突变基因的吸烟者患肺癌的风险度显著增加（OR=21．46，95％CI 4．64—99．20，P〈0．01和0R=7．60，95％CI=2．77～20．84，P〈0．01）。结论徐州地区汉族人群CYPIB1基因rs1056836位点、GSTP1基因rs1695位点单核苷酸多态性与肺癌易感性相关，两个位点单核苷酸多态性均是肺癌的风险基因，并且两个基因位点具有协同作用，风险基因越多患病风险越大；吸烟与两基因多态性之间有相互协同效应。

Objective To investigate the correlation between single nucleotide polymorphisms in the CYPIB1 rs1056836 and GSTP1 rs1695 and susceptibility to lung cancer among Han population in Xuzhou. Methods A case - control study was performed where 100 lung cancer patients who were pathologically diagnosed and 100 healthy control individuals who were selected from the Center of Health Examination of our hospital were enrolled. The genetic polymorphisms of CYPIBI rs1056836 and GSTP1 rs1695 were examined by real - time PCR. The correlation between the two genetic polymorphisms and susceptibility to human lung cancer was analyzed by smoking status. Results The individuals who carried with CYP1B1 rs1056836 and GSTP1 rs1695 mutant heterozygote and homozygote had a high risk of lung canc- er （ OR = 4.16, 3.77 and OR = 2.90, 3.04）. Polymorphisms at two loci presented a synergic effect （ OR = 11.67, 95 % CI 2.41 -56. 48, P 〈0.01 ）. The combined CYP1B1 rs1056836 and GSTP1 rs1695 mutant genotype and smoking were found to be significant risk factors of lung cancer （ OR = 21.46, 95 % CI 4. 64 - 99. 20, P 〈 0.01 and OR = 7.60, 95 % CI 2.77 -20.84, P〈0.01）. Conclusions Genetic polymorphisms of CYPIB1 rs1056836 and GSTP1 rs1695 are associated with lung cancer susceptibility among Han population in Xuzhou. Both genetic polymorphisms are the risk factors of lung cancer and work in a synergy manner. The more risk genes are, the more serious the risks are. Smoking works in a synergie manner with CYPIB1 rs1056836 and GSTP1 rs1695 genetic polymorphisms.