高原环境寻常型银屑病Th17、Th22、Treg细胞相关因子分析

Analysis of Th17,Th22,Treg cytokines in patients with psoriasis vulgaris in plateau

目的:探讨辅助性T细胞(Th17、Th22)及调节性T细胞(Treg细胞)相关因子在新疆高寒、高海拔、低氧环境下寻常型银屑病(psoriasis vulgaris,PV)患者中的表达水平。方法:选取37例新疆高原环境下PV患者(高原PV组)、40例非高原环境下PV患者(非高原PV组)及20例健康对照者(对照组)为研究对象,用酶联免疫吸附法检测患者血清中白介素-17(IL-17)、白介素-6(IL-6)、白介素-22(IL-22)、白介素-10(IL-10)及转化生长因子β1(TGF-β1)的水平,并进行比较分析。结果:高原PV组及非高原PV组血清IL-17、IL-6、IL-22的水平均显著高于对照组(P<0.01),且高原PV组明显高于非高原PV组,差异有统计学意义(P<0.05);高原PV组和非高原PV组IL-10、TGF-β1水平明显低于对照组(P<0.01),且高原PV组明显低于非高原PV组,差异有统计学意义(P<0.05)。皮损面积严重指数(psoriasis area and severity index,PASI)评分与外周血清IL-17、IL-22、IL-6水平呈正相关,与IL-10、TGF-β1呈负相关(P<0.01或P<0.05)。结论:新疆高原环境和非高原环境中PV患者血清IL-17、IL-6、IL-22的水平增高,并与PASI评分呈正相关,IL-10及TGF-β1的水平降低,并与PASI评分呈负相关。Th17、Th22、Treg细胞因子可能参与高原环境PV的发病及进展。

Objective：To explore the levels of serum Th17,Th22,Treg cytokines in patients with psoriasis vulgaris（PV）in Xinjiang plateau.Methods：Thirty-seven PV patients in Xinjiang plateau（PV plateau group）,40 PV patients in non-plateau region（PV non-plateau group）and 20 healthy people（control group）were enrolled in this study.The levels of serum IL-17,IL-6,IL-22,IL-10,TGF-β1 were detected by ELISA and were compared and analyzed.Results：The levels of serum IL-17,IL-6,IL-22 were increased compared with control group（P〈0.01）,and levels of the PV plateau group were significantly higher than those of PV non-plateau group（P〈 0.05）.The levels of IL-10 and TGF-β1 of the PV patients were decreased compared with control group（P〈 0.01）,and levels of the PV plateau group were significantly lower than those of PV non-plateau group（P〈0.05）.The levels of IL-17,IL-22 and IL-6 were positively correlated with psoriasis area and severity index（PASI）scores（P〈0.01 or P〈0.05）.The levels of IL-10 and TGF-β1 were negative correlated with PASI scores（P〈0.01 or P〈0.05）.Conclusion：The levels of serum IL-17,IL-6,IL-22 are lower in PV patients in Xinjiang plateau and in non-plateau region,and they are positively correlated with PASI scores.The levels of serum IL-10 and TGF-β1 decreased in PV patients in Xinjiang plateau and non-plateau region,and they are negtively correlated with PASI scores.The abnormal expression of Th17,Th22,Treg cytockins and Treg cells may play a critical role in pathogenesis and progression of psoriasis.