摘要
利用高效液相色谱—蒸发光检测法(HPLC-ELSD)建立基于指纹图谱分析的低聚半乳糖成分比较与组分含量推算方法。采用该方法对11个匿名食品级低聚半乳糖产品进行含量分析与比较。研究结果表明,现阶段食品级低聚半乳糖产品组成接近,非有效组分主要由葡萄糖、半乳糖和乳糖构成;有效组分色谱峰个数1~2个,质量浓度范围为73.65~100.00g/100g,主要由聚合度大于3的低聚半乳糖混合构成,部分产品中有效组分仅为异乳糖。通过该方法的建立与应用,可实现对低聚半乳糖产品质量差异的有效评价。
In this study,the High Performance Liquid Chromatography-Evaporative Light Detection（HPLC-ELSD） was used to establish the rapid comparison and content calculation of effective components of galacto-oligosaccharides based on the fingerprint analysis.Using this method,11 anonymous commercial food-grade galacto-oligosaccharides products were compared based on the fingerprint composition comparison and content calculation of the effective component.The results showed that the composition of the commercial food-grade galacto-oligosaccharides product composition close to each other,and the non-effective components generally included glucose,galactose and lactose constitute.The number of effective ingredient peaks was 1 to 2,and the mass concentration range was 73.65～100.00 g/100 g.It was mainly composed of a mixture of galacto-oligosaccharides with a degree of polymerization greater than 3,and the effective component in some products was exclusively iso-lactose.The establishment of this method has enabled the effective evaluation of the quality of galacto-oligosaccharides products.
作者
董艺凝
陈卫
邓文君
陈海琴
DONG Yi-ning1, CHEN Wei2, DENG Wen-jun1, CHEN Hai-qin2(1. College of Biological Science and Food Engineering, Chuzhou University, Chuzhou, Anhui 239000, China; 2. College of Food Science and Technology, Yiangnan University, Wuxi, jiangsu 214122, Chin)
出处
《食品与机械》
CSCD
北大核心
2018年第7期54-58,共5页
Food and Machinery
基金
国家自然科学基金青年项目(编号:31301523)
安徽省自然科学基金面上项目(编号:1708085MC72)
滁州学院食品酶法加工科技创新团队(编号:00001702)
高校优秀青年骨干人才国内访学研究项目
教育部中西部高等学校青年骨干教师国内访学项目(编号:gxfx2017120)
关键词
低聚半乳糖
指纹图谱
高效液相色谱—蒸发光检测法
含量推算
有效组分
galacto-oligosaccharides ； fingerprint
high performance liquid chromatography-evaporative light detection （ HPLC-ELSD）； content calculation； effective components
