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Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2 被引量:7

解脲脲原体脂质相关膜蛋白经Toll样受体2信号通路调控人羊膜上皮细胞诱导IL-6、IL-8和TNF-α的产生(英文)
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摘要 Objective: The purpose of this study was to determine the role of Ureaplasma urealyticum-derived lipidassociated membrane proteins (LAMPs) in the host innate immune system, specifically their effect on Toll-like receptors (TLRs). Methods: LAMPs were derived from U. urea/yticum strains, and human amniotic epithelial cells (HAECs) were isolated from healthy full-term placentas. Cytokine concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and TLR2 mRNA by real-time PCR. Expression of TLR2 was confirmed by Western blotting and immunohistochemistry. Results: LAMPs induced HAECs to produce inflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α. Cytokine production was reduced after blocking TLR2 using TLR2 inhibitor (anti-hTLR2-IgA). Conclusions: LAMPs isolated from U. urealyticum induced TLR2-dependent up-regulation of inflammatory genes and cytokines in HAECs. 目的:探讨解脲脲原体(Ureaplasma urealyticum)及其脂质相关膜蛋白(LAMPs)作用于人羊膜上皮细胞(HAECs)过程中白介素6(IL-6)、IL-8和肿瘤坏死因子α(TNF-α)的变化情况,阐明Toll样受体2(TLR2)的调控机制,明确解脲脲原体潜在的致病性。创新点:从解脲脲原体诱导炎症反应的分子机制入手,提出TLR2信号通路在其中的关键作用。方法:经TX-114处理萃取解脲脲原体获得LAMPs,将LAMPs和解脲脲原体分别感染HAECs,用酶联免疫吸附试验(ELISA)测定炎症细胞因子(IL-6、IL-8和TNF-α);采用实时聚合酶链反应(real-time PCR)测定TLR2 mRNA水平,用蛋白质印迹(Western blot)检测TLR2的表达量;经TLR2阻断剂(anti-h TLR2-IgA)处理后,测定相应炎症细胞因子。结论:解脲脲原体LAMPs能诱导HAECs的TLR2表达上调和炎症因子增加,从而发生炎症反应;TLR2受阻断后,炎症因子表达减少,炎症水平下降。TLR2在解脲脲原体LAMPs感染HAECs过程起关键作用。
出处 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2018年第8期654-661,共8页 浙江大学学报(英文版)B辑(生物医学与生物技术)
基金 Project supported by the Zhejiang Provincial Natural Science Foundation of China(Nos.LY18H040001 and LY16H040003) the Department of Education of Zhejiang Province(No.Y201534723) the Science and Technology Development Project in Hangzhou(No.20160533B13),China
关键词 Ureaplasma urealyticum Lipid-associated membrane protein Human amniotic epithelial cell Toll-like receptor 2 解脲脲原体 脂质相关膜蛋白 人羊膜上皮细胞Toll样受体2
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