冠状动脉疾病患者血清IL-12、IL-35水平与ox-LDL及冠状动脉狭窄程度的关系

Associations of serum IL-12 and IL-35 levels with serum ox-LDL level and degree of coronary artery stenosis in patients with coronary artery disease

目的分析冠状动脉疾病患者血清白细胞介素12(IL-12)、白细胞介素35(IL-35)水平与氧化型低密度脂蛋白(ox-LDL)及冠状动脉狭窄严重程度的关系。方法选择2016年9月至2017年12月诊治的75例冠状动脉疾病患者,其中急性心肌梗死(AMI) 28例,不稳定性心绞痛(UAP) 25例,稳定性心绞痛(SAP) 22例。所有患者均行冠状动脉造影,采用冠状动脉狭窄程度积分(Gensini积分)法评价病例组患者冠状动脉狭窄程度,根据Gensini积分分为≥30分组和<30分组。另选同时期体检健康者24例作为对照组。采用酶联免疫吸附法(ELISA)检测各组血清IL-12、IL-35和ox-LDL水平,Pearson相关分析法分析冠状动脉疾病患者血清IL-12、IL-35水平与ox-LDL水平和Gensini积分的相关性。结果随冠状动脉病变程度加重(对照组→SAP组→UAP组→AMI组),IL-12、oxLDL、Gensini积分逐渐升高,IL-35水平逐渐下降,各组间两两比较差异均有统计学意义(P <0. 05); Gensini积分≥30分组血清IL-12和ox-LDL水平显著高于Gensini积分<30分组(P <0. 01),血清IL-35水平显著低于Gensini积分<30组(P <0. 01);冠状动脉疾病患者血清IL-12水平与ox-LDL水平呈显著正相关(r=0. 848,P=0. 000),与Gensini积分呈显著正相关(r=0. 826,P=0. 000);冠状动脉疾病患者血清IL-35与ox-LDL呈显著负相关(r=-0. 806,P=0. 000),与Gensini积分呈显著负相关(r=-0. 745,P=0. 000)。结论血清IL-12、IL-35与ox-LDL参与冠状动脉疾病发生发展,IL-12、IL-35与冠状动脉狭窄严重程度密切相关,可作为评价冠状动脉狭窄严重程度的参考指标。

Objective To analyze the associations of serum interleukin（IL）-12 and IL-35 levels with serum oxidized low density lipoprotein（ox-LDL） level and degree of coronary artery stenosis in patients with coronary artery disease. Methods Seventy-five patients with coronary heart disease（CHD） including 28 cases of acute myocardial infarction（group AMI）,25 cases of unstable angina pectoris（group UAP） and 22 cases of stable angina pectoris（group SAP） diagnosed and treated from September 2016 to December 2017 were selected. Coronary angiography was performed in all patients. The degree of coronary artery stenosis was evaluated with Gensini integral,and the CHD patients were divided into Gensini integral more than or equal to 30 group and Gensini integral less than 30 group according to Gensini integral. Twenty-four healthy subjects at the same time were selected as control group. Enzyme linked immunosorbent assay（ELISA） was used to detect serum IL-12,IL-35 and ox-LDL levels. Pearson correlation analysis was used to analyze the correlations of serum IL-12 and IL-35 levels with serum ox-LDL level and Gensini integral in CHD patients. Results With the increase of coronary artery lesion degree in the order of control group,group SAP,group UAP,group AMI,the serum IL-12 and ox-LDL levels and Gensini integral increased gradually,and IL-35 level decreased gradually,and there were statistical differences in them for pairwise comparison in groups（all P〈0. 05）. In Gensini integral more than or equal to 30 group,serum IL-12 and oxLDL levels were significantly higher than those in Gensini integral less than 30 group（all P〈0. 05）,and serum IL-35 level was significantly lower than that in Gensini integral less than 30 group（P〈0. 05）. In CHD patients,serum IL-12 level were positively correlated with serum ox-LDL level（r = 0. 848,P = 0. 000） and Gensini integral（r = 0. 826,P = 0. 000）,and serum IL-35 level were negatively correlated with serum ox-LDL level（r =-0. 806,P = 0. 000） and Gensini integral（r =-0. 745,P = 0. 000）. Conclusion IL-12,IL-35 and ox-LDL participate in the occurrence and development of CHD.Serum IL-12 and IL-35 are closely related to the severity of coronary artery stenosis,and they can be used as the reference indexes to evaluate the severity of coronary artery stenosis.