城镇排水内分泌干扰物综合毒性效应的CALUX检测方法

Detection of Comprehensive Toxicity Effect of Endocrine Disruptors in Urban Drainage by CALUX Bioassay

针对当前城镇水环境中日益严重的内分泌干扰物(EDCs)污染,参照已取得ISO17025认证、美国环保局(EPA)批准的荧光素酶表达基因法(CALUX),研究建立了污水和污泥中二口恶英、雌激素、雄激素、肾上腺皮质激素等四类主要EDCs的综合毒性效应检测方法,并通过重复试验对方法的精密度进行了验证。结果表明,CALUX方法作为一种EDCs综合毒性效应的快速生物检测方法,具有较好的灵敏度、重现性、可靠性,可作为仪器分析的有效补充方法。

In view of the increasing pollution of endocrine disruptors （EDCs） in the urban water environment, the Chemical-Lueiferase Gene Expression （CALUX） test method, which was certified by the International Organization for Standardization （ISO 17025 ） and approved by the U. S. Environmental Protection Agency （EPA）, was taken as a reference to establish the detection method for comprehensive toxicity of the increasingly serious pollution of endocrine disrupting chemicals （EDCs） in water environment, biological screening assay of EDCs including dioxin, estrogen, androgen and adrenaline in sewage and sludge. The precision and standard addition recovery of the method was verified. The results showed that CALUX bioassay was a rapid biological method with good sensitivity, reproducibility and maneuver- ability for comprehensive toxicity effect screening of EDCs. CALUX bioassay could be used as an effective supplement to the classic instrument analysis method.