仿刺参体壁提取物海参糖胺聚糖对肺结核患者外周血体外细胞免疫调节功能的影响

Study on glycosaminoglycan from Apostichopus Japonius on cellular immuno-regulation function of peripheral blood cells from pulmonary tuberculosis patients in vitro

目的探讨仿刺参体壁提取物海参糖胺聚糖(HGAG)对肺结核患者外周血体外细胞免疫功能影响。方法选取健康人群40例(健康组)和肺结核患者30例(结核组)抗凝外周血4 ml,分离外周单个核血细胞(PBMC),与HGAG体外共培养24 h。采用流式细胞仪检测CD45RA、CD45RO、CD1a和CD83的表达。结果肺结核组CD45RA和CD45RO表达以HGAG浓度为50μg/ml最明显(P <0.05);而健康组CD45RA、CD45RO表达分别以10μg/ml、50μg/ml HGAG浓度最明显(P <0.001)。培养前两组间CD45RA表达差异无统计学意义(P>0.05),CD45RO表达比较差异有统计学意义(P <0.01);培养后两组CD45RA和CD45RO在10μg/ml和50μg/ml HGAG时表达差异有统计学意义(P <O.05)。健康组在培养前后,CD1a和CD83表达差异无统计学意义(P> 0.05),而结核组在培养前后比较,CD1a和CD83表达差异均有统计学意义(P <0.05)。健康组与肺结核组在培养前CD1a表达差异无统计学意义(P> 0.05),而CD83表达差异有统计学意义(P <0.001);培养后,两组间CD1a和CD83表达差异均无统计学意义(P>0.05)。结论 HGAG在一定浓度范围内可以下调CD45RA表达和上调CD45RO表达,并促进肺结核患者树突状细胞(DC)成熟,从而调节肺结核患者的细胞免疫。

Objective To investigate the effects of glycosaminoglycans （HGAG） on the immune function of peripheral blood cells from patients with pulmonary tuberculosis. Methods Peripheral blood monouclear cells （PBMC） were isolated from peripheral blood of 40 healthy people （ healthy group） and 30 tuberculosis patients （ tuberculosis group） and coeuhured with HGAG in vitro for 24 hours. Flow cytometry was used to detect the expression of CIM5RA and CD45RO, as well as the expression of CDla and CD83. Results The results showed that the expression of CD45RA and CD45RO in the tuberculosis group was the most significant （ P 〈 0. 05 ） at the concentration of 50 μg/ml coculturing with HGAG. The expression of CD45RA and CD45RO were most obvious in the healthy group at the concentration of 10 μg/ml and 50 μg/ml respectively （P〈0. 001）. The difference of CD45RA between the two groups was no significant （P〉 0. 05 ）, while the difference of CD45RO was statistically significant （ P 〈 0. 01 ） before co-culturing. The expression of CD45RA and CD45RO at 10 μg/ml and 50 μg/ml after co-culturing with HGAG were statisti- cally significant （P 〈 0. 05 ）. There was no statistical difference in CDla and CD83 in healthy group beforeand after co-culturing （ P 〉 0. 05 ） , while there was statistically difference （ P 〈 0. 05 ） before and after culturing in tuberculosis group. Before co-culturing, there was no significant difference in the expression of CD1 a between the healthy group and the tuberculosis group （P 〉 0.05 ）, but CD83 expression was statistically different （P 〈 0. 001 ）. After co-culturing, there were no significant differences in CD1 a and CD83 expression between healthy and healthy groups （P 〉 0. 05）. Conclusions HGAG can down-regulate the expression of CIM5RA and up-regulate the expression of CIM5RO in a certain concentration range, and promote the maturation of dendritic cells （DC） in tuberculosis patients and regulate the cellular immunity of patients with pulmonary tuberculosis in vitro.