内皮抑素30肽对HepG2细胞增殖活性和黏附能力的影响

The influence of Endostatin 30 peptide on proliferative activity and adhesion ability of HepG2 cell

目的探讨内皮抑素30肽对肝癌HepG2细胞增殖活性和黏附能力的影响。方法将内皮抑素氨基端的1～31位氨基酸(将25～31位序列由RGIRGAD改为RGDRGD)对应的核苷酸序列连接到质粒pTYB2中,再转化至大肠埃希菌BL21(DE3)中表达,合成由30个氨基酸构成的30肽。结果 HepG2细胞经0、20、40、60、80、100μg/ml的30肽分别处理24、48 h后,细胞的增殖活性和黏附能力均被抑制,呈时间剂量依赖性,与对照组比较,差异有统计学意义(P<0.05)。结论内皮抑素30肽能有效抑制HepG2细胞的增殖和黏附,在临床上有望成为肝癌的辅助治疗手段之一。

Objective To investigate the influence of Endostatin 30 peptide on proliferative activity and adhesion abili- ty of HepG2 cell for liver cancer. Methods The nueleotide sequences corresponding to amino-terminal 1-31 amino acids of endostatin （change the 25-31 bit sequence fi＇om RGIRGAD to RGDRGD） were linked to plasmid pTYB2, then converted to the expression of Escherichia coli BL21 （DE3） and synthesize 30 peptides composed of 30 amino acids. Results Afier HepG2 cells were treated by 30 peptide of 0, 20, 40, 60, 80, 100 μg/ml for 24, 48 h, the proliferative activity and adhesion of cells were inhibited in time and dose dependent, and compared with the control group, there was a significant difference （P〈0.05）. Conclusion Endostatin 30 peptide can effectively inhibit the proliferation and adhesion of HepG2 cell, which is expected to become one of the adjuvant therapies for liver cancer.