摘要
核桃腐烂病的发生严重影响核桃树的生长与结果。将染病树皮进行培养,划线分离出一株真菌,应用PCR技术对分离菌株的线粒体基因[the small r RNA subunit(rns)]克隆后测序,并将所测序列与美国国家生物技术信息中心(Natinal Center for Biotechnology Information,NCBI)数据库中已有的序列进行分析比较,利用Neighbor-joining建立系统发育树。结果表明,分离出的菌株与茎点霉属(Phoma sp.)聚集在同一支上,可以确定是茎点霉属(Phoma sp.)。
Walnut rot was a serious disease which brought damage to growing and fruiting of walnut tree. A strain of fungus was isolated from cultivating infected bark of walnut tree, and its small r RNA subunit was cloned and sequenced by PCR. The sequces were analyzed and compared with the existing sequences in NCBI, and phylogenetic tree was established with Neighbor-joining. The results showed that the isolated strain had high homology with Phoma sp.（97%）,and it was proved that strain was belonged to Phoma sp.
作者
张海军
陈春艳
谢映平
田忠科
Zhang Haijun;Chen Chunyan;Xie Yingping;Tian Zhongke(Yangquan Forestry Sciences Research Institute,Yangquan Shanxi 045000,China;College of Life Sciences,Shanxi University,Taiyuan Shanxi 030006,China;Yangquan Applied Technology Institute,Yangquan Shanxi 045000,China)
出处
《中国植保导刊》
北大核心
2018年第9期17-20,共4页
China Plant Protection
基金
山西省科技攻关项目(20140311017-6)
关键词
核桃腐烂病
病原真菌
分离
鉴定
茎点霉属
walnut rot disease
pathogen fungus
isolation
identification
Phoma sp.
