摘要
Objective： To evaluate the effect of Heyan Kuntai Capsule （和颜坤泰胶囊, HYKT） on the ovarian function of aged mice and expressions of cohesion complexes in oocytes. Methods： Twenty-five 9-month-old female C57BL/6J mice were randomly divided into 5 groups by block randomization method （n=5 per group）, including the control group （saline）, 17β-estradiol group [E2, 100 μg/（kg·d）], and low-, medium-, and high- dose of HYKT groups [0.3, 0.9, 2.7 g/（kg·d）, respectively]. All mice were treated by intragastric administration for 4 weeks. Hematoxylin and eosin staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-gamma H2A histone family member X （γ, H2AX） staining and TdT-mediated dUTP Nick-End Labeling （TUNEL） assay. The density of cohesin subunits, REC8 meiotic recombination protein （REC8）, structural maintenance of chromosome （SMC） 1 β and SMC3 in oocytes were evaluated by immunofluorescent staining. Results： After administration of E2 and high-dose of HYKT, the total number of follicles as well as the number of primordial and primary follicles were significantly increased （P〈0.05）. Anti-γ/H2AX staining and TUNEL assay demonstrated that high-dose of HYKT and E2 partly suppressed the apoptosis of follicles （P〈0.05）. Furthermore, it showed an increased trend in the levels of REC8 and SMC1 β, after administration with E2 and HYKT, and no obvious change in the level of SMC3. Conclusion： HYKT could enhance the number of follicles, suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits （REC8 and SMC1 β） in oocytes of aged mice, indicating a beneficial effect on the ovarian function in terms of the quantity and quality of follicles.
Objective： To evaluate the effect of Heyan Kuntai Capsule （和颜坤泰胶囊, HYKT） on the ovarian function of aged mice and expressions of cohesion complexes in oocytes. Methods： Twenty-five 9-month-old female C57BL/6J mice were randomly divided into 5 groups by block randomization method （n=5 per group）, including the control group （saline）, 17β-estradiol group [E2, 100 μg/（kg·d）], and low-, medium-, and high- dose of HYKT groups [0.3, 0.9, 2.7 g/（kg·d）, respectively]. All mice were treated by intragastric administration for 4 weeks. Hematoxylin and eosin staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-gamma H2A histone family member X （γ, H2AX） staining and TdT-mediated dUTP Nick-End Labeling （TUNEL） assay. The density of cohesin subunits, REC8 meiotic recombination protein （REC8）, structural maintenance of chromosome （SMC） 1 β and SMC3 in oocytes were evaluated by immunofluorescent staining. Results： After administration of E2 and high-dose of HYKT, the total number of follicles as well as the number of primordial and primary follicles were significantly increased （P〈0.05）. Anti-γ/H2AX staining and TUNEL assay demonstrated that high-dose of HYKT and E2 partly suppressed the apoptosis of follicles （P〈0.05）. Furthermore, it showed an increased trend in the levels of REC8 and SMC1 β, after administration with E2 and HYKT, and no obvious change in the level of SMC3. Conclusion： HYKT could enhance the number of follicles, suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits （REC8 and SMC1 β） in oocytes of aged mice, indicating a beneficial effect on the ovarian function in terms of the quantity and quality of follicles.
基金
Supported by the National Natural Science Foundation of China(No.31571196,81401171,30801502)
the 2015 Program to Guide Medicine of the Shanghai Municipal Science and Technology Commission(No.15401932200)
the FY2008 JSPS Postdoctoral Fellowship for Foreign Researchers(No.P08471)
the Shanghai Pujiang Program(No.11PJ1401900)
the Development Project of Shanghai Peak Disciplines-Integrated Chinese and Western Medicine
the Program for Outstanding Medical Academic Leader
Development Project of Shanghai Peak Disciplines-Integrated Chinese and Western Medicine(No.20150407)
